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MED30 mediator complex subunit 30 [ Homo sapiens (human) ]

Gene ID: 90390, updated on 5-Mar-2024

Summary

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Official Symbol
MED30provided by HGNC
Official Full Name
mediator complex subunit 30provided by HGNC
Primary source
HGNC:HGNC:23032
See related
Ensembl:ENSG00000164758 MIM:610237; AllianceGenome:HGNC:23032
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
MED30S; THRAP6; TRAP25
Summary
The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010]
Expression
Ubiquitous expression in bone marrow (RPKM 9.2), lymph node (RPKM 5.0) and 24 other tissues See more
Orthologs
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Genomic context

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Location:
8q24.11
Exon count:
5
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 8 NC_000008.11 (117520713..117540262)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 8 NC_060932.1 (118648865..118668421)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 8 NC_000008.10 (118532952..118552501)

Chromosome 8 - NC_000008.11Genomic Context describing neighboring genes Neighboring gene solute carrier family 30 member 8 Neighboring gene uncharacterized LOC105375716 Neighboring gene RNA, 7SL, cytoplasmic 826, pseudogene Neighboring gene uncharacterized LOC105375717 Neighboring gene NANOG hESC enhancer GRCh37_chr8:118369992-118370493 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr8:118400318-118401517 Neighboring gene ribosomal protein S10 pseudogene 16 Neighboring gene uncharacterized LOC105375721 Neighboring gene uncharacterized LOC107986970

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells. Syring I, et al. Ann Diagn Pathol, 2018 Jun. PMID 29661722
  2. The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer. Syring I, et al. Anticancer Res, 2017 Dec. PMID 29187445
  3. Mediator subunits: gene expression pattern, a novel transcript identification and nuclear localization in human endothelial progenitor cells. Rienzo M, et al. Biochim Biophys Acta, 2010 Jul. PMID 20493979
  4. Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s. Baek HJ, et al. Mol Cell Biol, 2002 Apr. PMID 11909976, Free PMC Article
  5. HIF1α and p53 Regulated MED30, a Mediator Complex Subunit, is Involved in Regulation of Glioblastoma Pathogenesis and Temozolomide Resistance. Shukla A, et al. Cell Mol Neurobiol, 2021 Oct. PMID 32705436

See all (54) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. HIF1alpha and p53 Regulated MED30, a Mediator Complex Subunit, is Involved in Regulation of Glioblastoma Pathogenesis and Temozolomide Resistance.
    Title: HIF1α and p53 Regulated MED30, a Mediator Complex Subunit, is Involved in Regulation of Glioblastoma Pathogenesis and Temozolomide Resistance.
  2. MED30 seems to be involved in the progression of the renal cell carcinoma (RCC).
    Title: The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells.
  3. Knockdown of MED30 led to reduction of the tumour parameters proliferation, migration, and invasion in the bladder cancer (BCa) cell lines.
    Title: The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer.
  4. analysis of a novel transcript of MED30, termed MED30 short (MED30s) generated by alternative splicing
    Title: Mediator subunits: gene expression pattern, a novel transcript identification and nuclear localization in human endothelial progenitor cells.
  5. Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s.
    Title: Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Two new susceptibility loci for Kawasaki disease identified through genome-wide association analysis.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Replication interactions

Interaction Pubs
Knockdown of mediator complex subunit 30 (MED30) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat SiRNA knockdown of d MED30 shows a modest inhibition in HIV-1 Tat-mediated transcription levels as compared to control PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC9890

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables nuclear receptor coactivator activity NAS
Non-traceable Author Statement
more info
 PubMed 
enables nuclear thyroid hormone receptor binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables nuclear vitamin D receptor binding NAS
Non-traceable Author Statement
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transcription coactivator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transcription coregulator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transcription coregulator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin protein ligase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA polymerase II preinitiation complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription elongation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of transcription initiation by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription initiation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in somatic stem cell population maintenance IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of core mediator complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of mediator complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
part_of ubiquitin ligase complex IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
mediator of RNA polymerase II transcription subunit 30
Names
TRAP/Mediator complex component TRAP25
putative mediator of RNA polymerase II transcription subunit 30
thyroid hormone receptor-associated protein 6
thyroid hormone receptor-associated protein complex 25 kDa component

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001282986.2 → NP_001269915.1  mediator of RNA polymerase II transcription subunit 30 isoform 2

    See identical proteins and their annotated locations for NP_001269915.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoofrm 1.
    Source sequence(s)
    AA812233, AC024329, AC087361, FM179284
    Consensus CDS
    CCDS64959.1
    UniProtKB/Swiss-Prot
    Q96HR3
    Related
    ENSP00000431051.1, ENST00000522839.1
    Conserved Domains (1) summary
    pfam11315
    Location:29 → 141
    Med30; Mediator complex subunit 30

  2. NM_001363182.2 → NP_001350111.1  mediator of RNA polymerase II transcription subunit 30 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC024329, AC087361, AY083305
    Conserved Domains (1) summary
    pfam11315
    Location:29 → 147
    Med30; Mediator complex subunit 30

  3. NM_080651.4 → NP_542382.1  mediator of RNA polymerase II transcription subunit 30 isoform 1

    See identical proteins and their annotated locations for NP_542382.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest isoform (1).
    Source sequence(s)
    AA812233, AC024329, AC087361, AY083305
    Consensus CDS
    CCDS6323.1
    UniProtKB/Swiss-Prot
    C6GKU9, Q96HR3
    Related
    ENSP00000297347.3, ENST00000297347.7
    Conserved Domains (1) summary
    pfam11315
    Location:29 → 176
    Med30; Mediator complex subunit 30

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000008.11 Reference GRCh38.p14 Primary Assembly

    Range
    117520713..117540262
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060932.1 Alternate T2T-CHM13v2.0

    Range
    118648865..118668421
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q96HR3.1 GenPept UniProtKB/Swiss-Prot:Q96HR3

Gene LinkOut

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