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LIPF lipase F, gastric type [ Homo sapiens (human) ]

Gene ID: 8513, updated on 11-Apr-2024

Summary

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Official Symbol
LIPFprovided by HGNC
Official Full Name
lipase F, gastric typeprovided by HGNC
Primary source
HGNC:HGNC:6622
See related
Ensembl:ENSG00000182333 MIM:601980; AllianceGenome:HGNC:6622
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
GL; HGL; HLAL
Summary
This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Expression
Restricted expression toward stomach (RPKM 9296.8) See more
Orthologs
all
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Genomic context

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Location:
10q23.31
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 10 NC_000010.11 (88664442..88678814)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 10 NC_060934.1 (89548553..89562923)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 10 NC_000010.10 (90424199..90438571)

Chromosome 10 - NC_000010.11Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC101929727 Neighboring gene renalase, FAD dependent amine oxidase Neighboring gene ATAC-STARR-seq lymphoblastoid active region 3721 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 3722 Neighboring gene vomeronasal 1 receptor 55 pseudogene Neighboring gene NANOG-H3K4me1 hESC enhancer GRCh37_chr10:90107282-90107945 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 3723 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 3724 Neighboring gene lipase family member J Neighboring gene ribosomal protein L7 pseudogene 34 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chr10:90462586-90463435 Neighboring gene N-ethylmaleimide-sensitive factor attachment protein, gamma pseudogene 1 Neighboring gene keratin 8 pseudogene 38 Neighboring gene lipase family member K

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Constitutive expression of human gastric lipase in Pichia pastoris and site-directed mutagenesis of key lid-stabilizing residues. Sams L, et al. Biochim Biophys Acta Mol Cell Biol Lipids, 2017 Oct. PMID 28694218
  2. Expression of active recombinant human gastric lipase in Nicotiana benthamiana using the CPMV-HT transient expression system. Vardakou M, et al. Protein Expr Purif, 2012 Jan. PMID 21945702
  3. Importance of the lid and cap domains for the catalytic activity of gastric lipases. Miled N, et al. Comp Biochem Physiol B Biochem Mol Biol, 2003 Sep. PMID 12941646
  4. Mechanisms of inhibition of triacylglycerol hydrolysis by human gastric lipase. Pafumi Y, et al. J Biol Chem, 2002 Aug 2. PMID 11940604
  5. Gastric cancer cell lines as models to study human digestive functions. Basque JR, et al. J Cell Biochem, 2001 Mar 26. PMID 11241664

See all (44) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. site-directed mutagenesis the role of three key residues (K4, E225, R229) involved in salt bridges stabilizing the lid domain that controls the access to the active site and is part of the interfacial recognition site. Their substitution has an impact on the pH-dependent activity of rHGL and its relative activities on medium and long chain triglycerides.
    Title: Constitutive expression of human gastric lipase in Pichia pastoris and site-directed mutagenesis of key lid-stabilizing residues.
  2. LIPFDGKA might serve as a potential possible biomarkers for diagnosis of gastric cancer, and their downregulation may bring new perspective into the investigation of gastric cancer prognosis
    Title: Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer.
  3. In patients with intrahepatic cholestasis of pregnancy, there was no elevation in LIPF mRNA in maternal circulation compared with controls.
    Title: Intrahepatic Cholestasis of Pregnancy: mRNAs for LIPF and ELOVL4 Genes Are Not Detectable in Circulating Maternal Plasma.
  4. Recombinant human gastric lipase (hGL) was transiently expressed in Nicotiana benthamiana leaves using the CPMV-HT expression system.
    Title: Expression of active recombinant human gastric lipase in Nicotiana benthamiana using the CPMV-HT transient expression system.
  5. insights into the domain movements
    Title: Molecular dynamics simulations of human and dog gastric lipases: insights into domain movements.
  6. down-regulated KLF4, CHGA, GPX3, SST and LIPF, together with up-regulated SERPINH1, THY1 and INHBA is an 8-gene signature for gastric cancer
    Title: An 8-gene signature, including methylated and down-regulated glutathione peroxidase 3, of gastric cancer.
  7. Observational study of gene-disease association. (HuGE Navigator)
    Title: A common haplotype in NAPEPLD is associated with severe obesity in a Norwegian population-based cohort (the HUNT study).
  8. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
    Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
  9. Clinical trial of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
    Title: Genetic correlates of olanzapine-induced weight gain in schizophrenia subjects from north India: role of metabolic pathway genes.
  10. identidfied the cap and the lid residues involved in the binding with the lipidic substrate
    Title: Importance of the lid and cap domains for the catalytic activity of gastric lipases.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC138477, MGC142271

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables lipid binding NAS
Non-traceable Author Statement
more info
 PubMed 
enables malate dehydrogenase activity IEA
Inferred from Electronic Annotation
more info
  
enables triglyceride lipase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables triglyceride lipase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables triglyceride lipase activity TAS
Traceable Author Statement
more info
 PubMed 
Process Evidence Code Pubs
involved_in lipid catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in malate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in triglyceride metabolic process NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cellular_component ND
No biological Data available
more info
  
located_in extracellular region IEA
Inferred from Electronic Annotation
more info
  
is_active_in intracellular membrane-bounded organelle IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
gastric triacylglycerol lipase
Names
gastric lipase
lipase, gastric
NP_001185757.1
NP_001185758.1
NP_001185759.1
NP_004181.1
XP_011538613.1
XP_054223025.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001198828.2 → NP_001185757.1  gastric triacylglycerol lipase isoform 3 precursor

    See identical proteins and their annotated locations for NP_001185757.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (3) is shorter at the N-terminus and lacks an internal segment compared to isoform 1.
    Source sequence(s)
    AK301310, BM797485
    Consensus CDS
    CCDS55719.1
    UniProtKB/TrEMBL
    Q53F21
    Related
    ENSP00000477140.1, ENST00000608620.5
    Conserved Domains (2) summary
    PLN02872
    Location:4 → 357
    PLN02872; triacylglycerol lipase
    pfam04083
    Location:35 → 82
    Abhydro_lipase; Partial alpha/beta-hydrolase lipase region

  2. NM_001198829.2 → NP_001185758.1  gastric triacylglycerol lipase isoform 1 precursor

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AK301320, BM797485, X05997
    Consensus CDS
    CCDS55718.1
    UniProtKB/TrEMBL
    Q53F21
    Related
    ENSP00000377900.3, ENST00000394375.7
    Conserved Domains (3) summary
    PLN02872
    Location:14 → 400
    PLN02872; triacylglycerol lipase
    pfam04083
    Location:45 → 107
    Abhydro_lipase; Partial alpha/beta-hydrolase lipase region
    pfam12695
    Location:88 → 211
    Abhydrolase_5; Alpha/beta hydrolase family

  3. NM_001198830.2 → NP_001185759.1  gastric triacylglycerol lipase isoform 4 precursor

    See identical proteins and their annotated locations for NP_001185759.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (4) lacks an internal segment compared to isoform 1.
    Source sequence(s)
    AL833751, BP341513
    Consensus CDS
    CCDS65896.1
    UniProtKB/TrEMBL
    Q53F21
    Related
    ENSP00000348101.3, ENST00000355843.2
    Conserved Domains (2) summary
    PLN02872
    Location:14 → 367
    PLN02872; triacylglycerol lipase
    pfam04083
    Location:45 → 92
    Abhydro_lipase; Partial alpha/beta-hydrolase lipase region

  4. NM_004190.4 → NP_004181.1  gastric triacylglycerol lipase isoform 2 precursor

    See identical proteins and their annotated locations for NP_004181.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.
    Source sequence(s)
    BM797485, BP341513, X05997
    Consensus CDS
    CCDS7389.1
    UniProtKB/Swiss-Prot
    B7Z723, F5H1P4, P07098, Q2M1P6, Q5VXI7, Q5VXI8, Q658L8
    UniProtKB/TrEMBL
    Q53F21
    Related
    ENSP00000238983.5, ENST00000238983.9
    Conserved Domains (3) summary
    PLN02872
    Location:4 → 390
    PLN02872; triacylglycerol lipase
    pfam04083
    Location:35 → 97
    Abhydro_lipase; Partial alpha/beta-hydrolase lipase region
    pfam12695
    Location:78 → 201
    Abhydrolase_5; Alpha/beta hydrolase family

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000010.11 Reference GRCh38.p14 Primary Assembly

    Range
    88664442..88678814
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011540311.2 → XP_011538613.1  gastric triacylglycerol lipase isoform X1

    See identical proteins and their annotated locations for XP_011538613.1

    UniProtKB/Swiss-Prot
    B7Z723, F5H1P4, P07098, Q2M1P6, Q5VXI7, Q5VXI8, Q658L8
    UniProtKB/TrEMBL
    Q53F21
    Conserved Domains (3) summary
    PLN02872
    Location:4 → 390
    PLN02872; triacylglycerol lipase
    pfam04083
    Location:35 → 97
    Abhydro_lipase; Partial alpha/beta-hydrolase lipase region
    pfam12695
    Location:78 → 201
    Abhydrolase_5; Alpha/beta hydrolase family

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060934.1 Alternate T2T-CHM13v2.0

    Range
    89548553..89562923
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054367050.1 → XP_054223025.1  gastric triacylglycerol lipase isoform X1

    UniProtKB/Swiss-Prot
    B7Z723, F5H1P4, P07098, Q2M1P6, Q5VXI7, Q5VXI8, Q658L8
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P07098.1 GenPept UniProtKB/Swiss-Prot:P07098

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