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TRIM45 tripartite motif containing 45 [ Homo sapiens (human) ]

Gene ID: 80263, updated on 3-Apr-2024

Summary

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Official Symbol
TRIM45provided by HGNC
Official Full Name
tripartite motif containing 45provided by HGNC
Primary source
HGNC:HGNC:19018
See related
Ensembl:ENSG00000134253 MIM:609318; AllianceGenome:HGNC:19018
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
RNF99
Summary
Predicted to enable ubiquitin-protein transferase activity. Predicted to be involved in positive regulation of transcription, DNA-templated. Predicted to act upstream of or within bone development. Located in cytosol; intercellular bridge; and nucleoplasm. [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in endometrium (RPKM 2.4), thyroid (RPKM 2.2) and 24 other tissues See more
Orthologs
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Genomic context

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Location:
1p13.1
Exon count:
8
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (117111060..117123927, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (117121483..117134348, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (117653682..117664371, complement)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene CD101 molecule Neighboring gene CD101 antisense RNA 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1578 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1236 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1579 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr1:117619501-117620700 Neighboring gene transcription termination factor 2 Neighboring gene microRNA 942 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1237 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1580 Neighboring gene uncharacterized LOC124904387 Neighboring gene MPRA-validated peak386 silencer Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr1:117689065-117690264 Neighboring gene ribosomal protein S15a pseudogene 9 Neighboring gene V-set domain containing T cell activation inhibitor 1 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:117714175-117714737 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:117714738-117715299

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. TRIM45 Suppresses the Development of Non-small Cell Lung Cancer. Peng X, et al. Curr Mol Med, 2020. PMID 31663468
  2. TRIM45 negatively regulates NF-κB-mediated transcription and suppresses cell proliferation. Shibata M, et al. Biochem Biophys Res Commun, 2012 Jun 22. PMID 22634006
  3. E3 ligase RNF99 negatively regulates TLR-mediated inflammatory immune response via K48-linked ubiquitination of TAB2. Zhang J, et al. Cell Death Differ, 2023 Apr. PMID 36681779, Free PMC Article
  4. TRIM45 functions as a tumor suppressor in the brain via its E3 ligase activity by stabilizing p53 through K63-linked ubiquitination. Zhang J, et al. Cell Death Dis, 2017 May 25. PMID 28542145, Free PMC Article
  5. TRIM45, a novel human RBCC/TRIM protein, inhibits transcriptional activities of ElK-1 and AP-1. Wang Y, et al. Biochem Biophys Res Commun, 2004 Oct 8. PMID 15351693

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.
    Title: TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.
  2. TRIM45 Suppresses the Development of Non-small Cell Lung Cancer.
    Title: TRIM45 Suppresses the Development of Non-small Cell Lung Cancer.
  3. These findings suggest that TRIM45 is a novel tumor suppressor that stabilizes and activates p53 in glioma.
    Title: TRIM45 functions as a tumor suppressor in the brain via its E3 ligase activity by stabilizing p53 through K63-linked ubiquitination.
  4. The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway.
    Title: The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway.
  5. These findings suggest that TRIM45 acts as a repressor for the NF-kappaB signal and regulates cell growth.
    Title: TRIM45 negatively regulates NF-κB-mediated transcription and suppresses cell proliferation.
  6. TRIM45 may act as a new transcriptional repressor in mitogen-activated protein kinase signaling pathway
    Title: TRIM45, a novel human RBCC/TRIM protein, inhibits transcriptional activities of ElK-1 and AP-1.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • FLJ13181

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin protein ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in bone development IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of inflammatory response IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein K48-linked ubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in intercellular bridge IDA
Inferred from Direct Assay
more info
  
is_active_in nucleoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  

General protein information

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Preferred Names
E3 ubiquitin-protein ligase TRIM45
Names
ring finger protein 99
NP_001139107.1
NP_079464.2
XP_047286870.1
XP_054194842.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_028008.1 RefSeqGene

    Range
    5041..15730
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001145635.2 → NP_001139107.1  E3 ubiquitin-protein ligase TRIM45 isoform 2

    See identical proteins and their annotated locations for NP_001139107.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
    Source sequence(s)
    AK222901, AY669488, BC034943, BU165629, EC541819
    Consensus CDS
    CCDS44200.1
    UniProtKB/TrEMBL
    Q5T2K3
    Related
    ENSP00000358476.3, ENST00000369464.7
    Conserved Domains (4) summary
    smart00557
    Location:391 → 479
    IG_FLMN; Filamin-type immunoglobulin domains
    cd00021
    Location:189 → 226
    BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.
    pfam00630
    Location:391 → 476
    Filamin; Filamin/ABP280 repeat
    cl23765
    Location:240 → 357
    iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits

  2. NM_025188.4 → NP_079464.2  E3 ubiquitin-protein ligase TRIM45 isoform 1

    See identical proteins and their annotated locations for NP_079464.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AK222901, AY669488, BU165629, EC541819
    Consensus CDS
    CCDS893.1
    UniProtKB/Swiss-Prot
    Q53GN0, Q5T2K4, Q5T2K5, Q8IYV6, Q9H8W5
    UniProtKB/TrEMBL
    Q5T2K3
    Related
    ENSP00000256649.4, ENST00000256649.9
    Conserved Domains (4) summary
    smart00557
    Location:399 → 497
    IG_FLMN; Filamin-type immunoglobulin domains
    cd00021
    Location:189 → 226
    BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.
    pfam00630
    Location:397 → 494
    Filamin; Filamin/ABP280 repeat
    cl23765
    Location:240 → 357
    iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

    Range
    117111060..117123927 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047430914.1 → XP_047286870.1  E3 ubiquitin-protein ligase TRIM45 isoform X1

    Related
    ENSP00000358473.3, ENST00000369461.3

RNA

  1. XR_007063980.1 RNA Sequence

  2. XR_246297.2 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060925.1 Alternate T2T-CHM13v2.0

    Range
    117121483..117134348 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054338867.1 → XP_054194842.1  E3 ubiquitin-protein ligase TRIM45 isoform X1

RNA

  1. XR_008486169.1 RNA Sequence

  2. XR_008486168.1 RNA Sequence

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9H8W5.2 GenPept UniProtKB/Swiss-Prot:Q9H8W5

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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