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BTF3 basic transcription factor 3 [ Homo sapiens (human) ]

Gene ID: 689, updated on 5-Mar-2024

Summary

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Official Symbol
BTF3provided by HGNC
Official Full Name
basic transcription factor 3provided by HGNC
Primary source
HGNC:HGNC:1125
See related
Ensembl:ENSG00000145741 MIM:602542; AllianceGenome:HGNC:1125
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
NACB; BTF3a; BTF3b; BETA-NAC
Summary
This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in ovary (RPKM 354.7), bone marrow (RPKM 186.5) and 25 other tissues See more
Orthologs
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Genomic context

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Location:
5q13.2
Exon count:
6
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 5 NC_000005.10 (73498442..73505667)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 5 NC_060929.1 (73979765..73986989)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 5 NC_000005.9 (72794267..72801492)

Chromosome 5 - NC_000005.10Genomic Context describing neighboring genes Neighboring gene long intergenic non-protein coding RNA 1386 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr5:72783409-72784169 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 22657 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr5:72794255-72795065 Neighboring gene BTF3 divergent transcript Neighboring gene FUN14 domain containing 2 pseudogene 1 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 16090 Neighboring gene ankyrin repeat family A member 2 Neighboring gene H3K27ac hESC enhancers GRCh37_chr5:72860724-72861278 and GRCh37_chr5:72861279-72861833

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. BTF3 promotes proliferation and glycolysis in hepatocellular carcinoma by regulating GLUT1. Wang P, et al. Cancer Biol Ther, 2023 Dec 31. PMID 37382415, Free PMC Article
  2. BTF3 confers oncogenic activity in prostate cancer through transcriptional upregulation of Replication Factor C. Zhang Y, et al. Cell Death Dis, 2021 Jan 5. PMID 33414468, Free PMC Article
  3. BTF3 promotes stemness and inhibits TypeⅠInterferon signaling pathway in triple-negative breast cancer. Wang H, et al. Biochem Biophys Res Commun, 2021 Jan 22. PMID 33383560
  4. Upregulation of BTF3 affects the proliferation, apoptosis, and cell cycle regulation in hypopharyngeal squamous cell carcinoma. Zhang Y, et al. Biomed Pharmacother, 2019 Oct. PMID 31404775
  5. Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved. Liu Q, et al. Cell Death Dis, 2019 Jul 1. PMID 31263147, Free PMC Article

See all (102) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Ubiquitination-specific protease 7 enhances stemness of hepatocellular carcinoma by stabilizing basic transcription factor 3.
    Title: Ubiquitination-specific protease 7 enhances stemness of hepatocellular carcinoma by stabilizing basic transcription factor 3.
  2. BTF3 promotes proliferation and glycolysis in hepatocellular carcinoma by regulating GLUT1.
    Title: BTF3 promotes proliferation and glycolysis in hepatocellular carcinoma by regulating GLUT1.
  3. BTF3-mediated regulation of BMI1 promotes colorectal cancer through influencing epithelial-mesenchymal transition and stem cell-like traits.
    Title: BTF3-mediated regulation of BMI1 promotes colorectal cancer through influencing epithelial-mesenchymal transition and stem cell-like traits.
  4. BTF3 confers oncogenic activity in prostate cancer through transcriptional upregulation of Replication Factor C.
    Title: BTF3 confers oncogenic activity in prostate cancer through transcriptional upregulation of Replication Factor C.
  5. miR802 inhibits the epithelialmesenchymal transition, migration and invasion of cervical cancer by regulating BTF3.
    Title: miR‑802 inhibits the epithelial‑mesenchymal transition, migration and invasion of cervical cancer by regulating BTF3.
  6. BTF3 promotes stemness and inhibits TypeInterferon signaling pathway in triple-negative breast cancer.
    Title: BTF3 promotes stemness and inhibits TypeⅠInterferon signaling pathway in triple-negative breast cancer.
  7. BTF3 is positively related to CRC and BTF3-siRNA attenuated the tumorigenicity of colorectal cancer cells via MAD2L2, MCM3 and PLK1 activity reduction.
    Title: Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved.
  8. Expression of BTF3 is upregulated in hypopharyngeal squamous cell carcinoma and this upregulation is positively correlated with lymph node metastasis of this malignancy.
    Title: Upregulation of BTF3 affects the proliferation, apoptosis, and cell cycle regulation in hypopharyngeal squamous cell carcinoma.
  9. Study showed that BTF3 is overexpressed in prostate cancer (PCa) tissues and correlates with stem-like traits. Cancer stem-like characteristics in PCa including self-renewal and metastatic potential were impaired by BTF3 loss. BTF3 could stabilize BMI1, a crucial regulator of prostate stem cell self-renewal. More importantly, data revealed that BTF3 is highly predictive of poor prognosis.
    Title: BTF3 sustains cancer stem-like phenotype of prostate cancer via stabilization of BMI1.
  10. TCGA analysis reveals high expression levels of BTF3 in luminal/ER + breast cancer and cell line models harboring ERalpha overexpression. Concordantly, BTF3 expression is highly and strongly associated with ESR1 expression in multiple breast cancer cohorts.
    Title: Inhibition of BTF3 sensitizes luminal breast cancer cells to PI3Kα inhibition through the transcriptional regulation of ERα.
Submit: New GeneRIF Correction See all GeneRIFs (18)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Replication interactions

Interaction Pubs
Knockdown of basic transcription factor 3 (BTF3) by shRNA library screening inhibits HIV-1 replication in cultured Jurkat T-cells PubMed

Protein interactions

Protein Gene Interaction Pubs
integrase gag-pol Using acetylated HIV-1 IN as bait in yeast two-hybrid screening identifies transcription regulatory and chromatin remodeling factors LEDGF/p75, KAP1, BTF3b, THRAP3, and HMGN2 as IN-binding partners PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables RNA binding HDA PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in in utero embryonic development IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of protein localization to endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
  
part_of nascent polypeptide-associated complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of nascent polypeptide-associated complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in nucleus ISS
Inferred from Sequence or Structural Similarity
more info
  

General protein information

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Preferred Names
transcription factor BTF3
Names
NAC-beta
RNA polymerase B transcription factor 3
nascent polypeptide-associated complex subunit beta
nascent-polypeptide-associated complex beta polypeptide

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001037637.2 → NP_001032726.1  transcription factor BTF3 isoform A

    See identical proteins and their annotated locations for NP_001032726.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (A).
    Source sequence(s)
    AC099522, BG182166, BU661989, BX359147
    Consensus CDS
    CCDS34185.1
    UniProtKB/Swiss-Prot
    A8K510, P20290, Q13893, Q76M56
    Related
    ENSP00000369965.3, ENST00000380591.8
    Conserved Domains (1) summary
    pfam01849
    Location:87 → 140
    NAC; NAC domain

  2. NM_001207.5 → NP_001198.2  transcription factor BTF3 isoform B

    See identical proteins and their annotated locations for NP_001198.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in translation initiation at a downstream AUG and a protein (isoform B) with a shorter N-terminus, compared to isoform A.
    Source sequence(s)
    AC099522, BC008062, BG182166, BU661989
    Consensus CDS
    CCDS4019.1
    UniProtKB/TrEMBL
    A0A7I2V3T6
    Related
    ENSP00000338516.8, ENST00000335895.12
    Conserved Domains (1) summary
    cd22055
    Location:9 → 125
    NAC_BTF3; basal transcription factor BTF3

  3. NM_001393652.1 → NP_001380581.1  transcription factor BTF3 isoform C

    Status: REVIEWED

    Source sequence(s)
    AC099522

  4. NM_001393653.1 → NP_001380582.1  transcription factor BTF3 isoform D

    Status: REVIEWED

    Source sequence(s)
    AC099522
    UniProtKB/TrEMBL
    A0A7I2YQL2
    Related
    ENSP00000503967.1, ENST00000676862.1

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000005.10 Reference GRCh38.p14 Primary Assembly

    Range
    73498442..73505667
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060929.1 Alternate T2T-CHM13v2.0

    Range
    73979765..73986989
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P20290.1 GenPept UniProtKB/Swiss-Prot:P20290

Gene LinkOut

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