ENO1 enolase 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: ENO1provided by HGNC
Official Full Name: enolase 1provided by HGNC
Primary source: HGNC:HGNC:3350
See related: Ensembl:ENSG00000074800 MIM:172430; AllianceGenome:HGNC:3350
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: NNE; PPH; MPB1; ENO1L1; ENO1-IT1; HEL-S-17
Summary: This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
Expression: Ubiquitous expression in kidney (RPKM 213.0), esophagus (RPKM 188.5) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 1p36.23

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (8861000..8878686, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (8394464..8412155, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (8921059..8938745, complement)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Multi-omics reveals the role of ENO1 in bladder cancer and constructs an epithelial-related prognostic model to predict prognosis and efficacy.
Title: Multi-omics reveals the role of ENO1 in bladder cancer and constructs an epithelial-related prognostic model to predict prognosis and efficacy.
Long non-coding RNA NMRAL2P promotes glycolysis and reduces ROS in head and neck tumors by interacting with the ENO1 protein and promoting GPX2 transcription.
Title: Long non-coding RNA NMRAL2P promotes glycolysis and reduces ROS in head and neck tumors by interacting with the ENO1 protein and promoting GPX2 transcription.
N6-methyladenosine methyltransferase KIAA1429 promoted ovarian cancer aerobic glycolysis and progression through enhancing ENO1 expression.
Title: N6-methyladenosine methyltransferase KIAA1429 promoted ovarian cancer aerobic glycolysis and progression through enhancing ENO1 expression.
Unrevealed roles of extracellular enolase-1 (ENO1) in promoting glycolysis and pro-cancer activities in multiple myeloma via hypoxia-inducible factor 1alpha.
Title: Unrevealed roles of extracellular enolase‑1 (ENO1) in promoting glycolysis and pro‑cancer activities in multiple myeloma via hypoxia‑inducible factor 1α.
Alpha-enolase 1 knockdown facilitates the proliferation and invasion of villous trophoblasts by upregulating COX-2.
Title: Alpha-enolase 1 knockdown facilitates the proliferation and invasion of villous trophoblasts by upregulating COX-2.
Keratin 17 covalently binds to alpha-enolase and exacerbates proliferation of keratinocytes in psoriasis.
Title: Keratin 17 covalently binds to alpha-enolase and exacerbates proliferation of keratinocytes in psoriasis.
miR-22-3p Suppresses Cell Proliferation and Migration of Gastric Cancer by Targeting ENO1.
Title: miR-22-3p Suppresses Cell Proliferation and Migration of Gastric Cancer by Targeting ENO1.
Overexpression of c-MYC Promoter Binding Protein-1 Enhances Proliferation and Glucose Metabolism of Melanoma Cells Lines.
Title: Overexpression of c-MYC Promoter Binding Protein-1 Enhances Proliferation and Glucose Metabolism of Melanoma Cells Lines.
The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation.
Title: The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation.
Glioblastoma glycolytic signature predicts unfavorable prognosis, immunological heterogeneity, and ENO1 promotes microglia M2 polarization and cancer cell malignancy.
Title: Glioblastoma glycolytic signature predicts unfavorable prognosis, immunological heterogeneity, and ENO1 promotes microglia M2 polarization and cancer cell malignancy.

Submit: New GeneRIF Correction See all GeneRIFs (146)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Identification of risk loci with shared effects on five major psychiatric disorders: a genome-wide analysis. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	Tandem affinity purification and mass spectrometry analysis identify alpha unit of enolase 1 (ENO1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Gag-Pol	gag-pol	Tandem affinity purification and mass spectrometry analysis identify alpha unit of enolase 1 (ENO1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Nef	nef	HIV-1 Nef downregulates the expression of enolase 1 (ENO1) protein in Nef-transfected SupT1 cells	PubMed
	nef	Tandem affinity purification and mass spectrometry analysis identify alpha unit of enolase 1 (ENO1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Pr55(Gag)	gag	Tandem affinity purification and mass spectrometry analysis identify alpha unit of enolase 1 (ENO1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Tat	tat	MBP-1 suppresses transcription from the HIV-1 LTR promoter, an effect that is inhibited by HIV-1 Tat, suggesting an important role for MBP-1 in the regulation of HIV-1 replication in infected cells	PubMed
retropepsin	gag-pol	Positional proteomics analysis identifies the cleavage of human enolase 1, alpha (ENO1) at amino acid residues 115-116 by the HIV-1 protease	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH64843 (e-PCR)
- UniSTS:90374

ksks293 (e-PCR)
- UniSTS:514334

RH104034 (e-PCR)
- UniSTS:98359

STS-X51804 (e-PCR)
- UniSTS:71235

99-13794 (e-PCR)
- UniSTS:240722

D10S2138 (e-PCR)
- UniSTS:55152

SHGC-57235 (e-PCR)
- UniSTS:42400

GDB:549140 (e-PCR)
- UniSTS:157611

RH93736 (e-PCR)
- UniSTS:89530

SHGC-74193 (e-PCR)
- UniSTS:37334

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables GTPase binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA binding	HDA more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables cadherin binding	HDA more info	PubMed
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables phosphopyruvate hydratase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphopyruvate hydratase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphopyruvate hydratase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphopyruvate hydratase activity	TAS Traceable Author Statement more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription corepressor activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription corepressor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in canonical glycolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in canonical glycolysis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in canonical glycolysis	TAS Traceable Author Statement more info
involved_in gluconeogenesis	TAS Traceable Author Statement more info
involved_in glycolytic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell growth	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of ATP biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of ATP biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of muscle contraction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of plasminogen activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to virus	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in M band	IEA Inferred from Electronic Annotation more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in extracellular exosome	HDA more info	PubMed
located_in extracellular space	HDA more info	PubMed
located_in membrane	HDA more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IC Inferred by Curator more info	PubMed
part_of phosphopyruvate hydratase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of phosphopyruvate hydratase complex	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: alpha-enolase; c-myc promoter-binding protein-1

Names: 2-phospho-D-glycerate hydro-lyase; ENO1 intronic transcript 1; ENO1 intronic transcript 1 (non-protein coding); MYC promoter-binding protein 1; alpha enolase like 1; enolase 1, (alpha); enolase-alpha; epididymis secretory protein Li 17; non-neural enolase; phosphopyruvate hydratase; plasminogen-binding protein; tau-crystallin

NP_001188412.1

EC 4.2.1.11

NP_001340275.1

EC 4.2.1.11

NP_001419.1

EC 4.2.1.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029470.1 RefSeqGene

Range

5407..23093

Download

GenBank, FASTA, Sequence Viewer (Graphics), LRG_1165

mRNA and Protein(s)

NM_001201483.4 → NP_001188412.1 c-myc promoter-binding protein-1 isoform MBP-1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs at the 5' end compared to variant 1, and initiates translation from a downstream in-frame start codon, resulting in a shorter isoform (MBP-1). This isoform is localized to the nucleus, and functions as a transcriptional repressor of c-myc protooncogene by binding to its promoter (PMID:20849415).

Source sequence(s)

AL139415, BC001810, GU170215

UniProtKB/TrEMBL

E2DRY6

Related

ENST00000464920.2

Conserved Domains (2) summary

PLN00191
Location:1 → 339

PLN00191; enolase

cd03313
Location:1 → 323

enolase; Enolase: Enolases are homodimeric enzymes that catalyse the reversible dehydration of 2-phospho-D-glycerate to phosphoenolpyruvate as part of the glycolytic and gluconeogenesis pathways. The reaction is facilitated by the presence of metal ions.
NM_001353346.3 → NP_001340275.1 alpha-enolase isoform 3

Status: REVIEWED

Source sequence(s)

AL139415, BC001810, DA820724, GU170215

Consensus CDS

CCDS85924.1

UniProtKB/TrEMBL

A0A2R8Y6G6

Related

ENSP00000495530.1, ENST00000647408.1

Conserved Domains (1) summary

PLN00191
Location:2 → 432

PLN00191; enolase
NM_001428.5 → NP_001419.1 alpha-enolase isoform 1

See identical proteins and their annotated locations for NP_001419.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes isoform (1), which is localized to the cytosol, and has alpha-enolase activity. It has been reported that the monomeric form of this isoform functions as a structural lens protein (tau-crystallin), and the dimeric form as an enolase (PMID:2462567).

Source sequence(s)

AK309177, BC021166

Consensus CDS

CCDS97.1

UniProtKB/Swiss-Prot

B2RD59, P06733, P22712, Q16704, Q4TUS4, Q53FT9, Q53HR3, Q658M5, Q6GMP2, Q71V37, Q7Z3V6, Q8WU71, Q96GV1, Q9BT62, Q9UCH6, Q9UM55

UniProtKB/TrEMBL

A0A024R4F1, A0A0K0K1K8

Related

ENSP00000234590.4, ENST00000234590.10

Conserved Domains (1) summary

PLN00191
Location:2 → 432

PLN00191; enolase