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DGKG diacylglycerol kinase gamma [ Homo sapiens (human) ]

Gene ID: 1608, updated on 11-Apr-2024

Summary

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Official Symbol
DGKGprovided by HGNC
Official Full Name
diacylglycerol kinase gammaprovided by HGNC
Primary source
HGNC:HGNC:2853
See related
Ensembl:ENSG00000058866 MIM:601854; AllianceGenome:HGNC:2853
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
DAGK3; DGK-GAMMA
Summary
This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Expression
Broad expression in brain (RPKM 8.0), heart (RPKM 3.6) and 15 other tissues See more
Orthologs
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Genomic context

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See DGKG in Genome Data Viewer
Location:
3q27.2-q27.3
Exon count:
25
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 3 NC_000003.12 (186147201..186362234, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 3 NC_060927.1 (188962845..189177970, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (185864990..186080023, complement)

Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC124909470 Neighboring gene ETS variant transcription factor 5 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr3:185815375-185815888 Neighboring gene ETV5 antisense RNA 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20931 Neighboring gene OCT4-NANOG-H3K4me1 hESC enhancer GRCh37_chr3:185826419-185827397 Neighboring gene uncharacterized LOC105374257 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:185911899-185912696 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:185927717-185928344 Neighboring gene NANOG-H3K4me1 hESC enhancer GRCh37_chr3:185933195-185933911 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20932 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:185939275-185940188 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14981 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20933 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20934 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20935 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20936 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20937 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14982 Neighboring gene ReSE screen-validated silencer GRCh37_chr3:185986639-185986811 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:186078791-186079308 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14983 Neighboring gene NANOG hESC enhancer GRCh37_chr3:186100205-186100706 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:186131925-186132644 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14984 Neighboring gene long intergenic non-protein coding RNA 2020 Neighboring gene long intergenic non-protein coding RNA 2052 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:186194185-186194957 Neighboring gene long intergenic non-protein coding RNA 2051

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Diacylglycerol kinase γ predicts prognosis and functions as a tumor suppressor by negatively regulating glucose transporter 1 in hepatocellular carcinoma. Guo Z, et al. Exp Cell Res, 2018 Dec 15. PMID 30399372
  2. Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer. Kai M, et al. Mol Carcinog, 2017 Jul. PMID 28218473
  3. Diacylglycerol kinase γ is a novel anionic phospholipid binding protein with a selective binding preference. Takeshita E, et al. Biochem Biophys Res Commun, 2014 Feb 21. PMID 24486543
  4. Nuclear transportation of diacylglycerol kinase gamma and its possible function in the nucleus. Matsubara T, et al. J Biol Chem, 2006 Mar 10. PMID 16407189
  5. Signaling roles of diacylglycerol kinases. Topham MK. J Cell Biochem, 2006 Feb 15. PMID 16288460

See all (33) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma.
    Title: Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma.
  2. Characterization of alpha-synuclein N-terminal domain as a novel cellular phosphatidic acid sensor.
    Title: Characterization of α-synuclein N-terminal domain as a novel cellular phosphatidic acid sensor.
  3. Decreased expression of DGKgamma in hepatocellular carcinoma tissues was an independent risk factor for a poor prognosis.
    Title: Diacylglycerol kinase γ predicts prognosis and functions as a tumor suppressor by negatively regulating glucose transporter 1 in hepatocellular carcinoma.
  4. Epigenetic silencing of DGKG expression is associated with skin cancer.
    Title: Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer.
  5. these results indicate that DGKG is an anionic phospholipid binding protein that preferably interacts with a small highly charged head group that is very close to the glycerol or sphingosine backbone.
    Title: Diacylglycerol kinase γ is a novel anionic phospholipid binding protein with a selective binding preference.
  6. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
    Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
  7. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
    Title: Implication of genetic variants near NEGR1, SEC16B, TMEM18, ETV5/DGKG, GNPDA2, LIN7C/BDNF, MTCH2, BCDIN3D/FAIM2, SH2B1, FTO, MC4R, and KCTD15 with obesity and type 2 diabetes in 7705 Chinese.
  8. Observational study of gene-disease association. (HuGE Navigator)
    Title: Analyses of shared genetic factors between asthma and obesity in children.
  9. the interaction of diacylglycerol kinase gamma with the Src homology 2 and C1 domains of beta2-chimaerin is induced synergistically by Phorbol ester and hydrogen peroxide
    Title: Phorbol ester and hydrogen peroxide synergistically induce the interaction of diacylglycerol kinase gamma with the Src homology 2 and C1 domains of beta2-chimaerin.
  10. diacylglycerol kinase gamma regulates macrophage differentiation.
    Title: Regulatory role of diacylglycerol kinase gamma in macrophage differentiation of leukemia cells.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association yields new sequence variants at seven loci that associate with measures of obesity.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Potential readthrough

Included gene: ETV5

Homology

Clone Names

  • MGC104993, MGC133330

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP-dependent diacylglycerol kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ATP-dependent diacylglycerol kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP-dependent diacylglycerol kinase activity TAS
Traceable Author Statement
more info
  
enables calcium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables lipid binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in diacylglycerol metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in diacylglycerol metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glycerolipid metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in intracellular signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in lipid phosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in lipid phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of protein kinase C signaling ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in neuron development IEA
Inferred from Electronic Annotation
more info
  
involved_in phosphatidic acid biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in phosphatidic acid biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in platelet activation TAS
Traceable Author Statement
more info
  
involved_in protein kinase C-activating G protein-coupled receptor signaling pathway IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of dendrite development IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane TAS
Traceable Author Statement
more info
  

General protein information

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Preferred Names
diacylglycerol kinase gamma
Names
DAG kinase gamma
diacylglycerol kinase, gamma 90kDa
diacylglyerol kinase gamma
diglyceride kinase gamma
NP_001074213.1
NP_001074214.1
NP_001337.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001080744.2 → NP_001074213.1  diacylglycerol kinase gamma isoform 2

    See identical proteins and their annotated locations for NP_001074213.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 2) that is shorter than isoform 1.
    Source sequence(s)
    AC112649, AC117414, BC089411, BM669549
    Consensus CDS
    CCDS43182.1
    UniProtKB/TrEMBL
    Q2M589
    Related
    ENSP00000339777.4, ENST00000344484.8
    Conserved Domains (7) summary
    smart00109
    Location:336 → 377
    C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)
    smart00045
    Location:553 → 727
    DAGKa; Diacylglycerol kinase accessory domain (presumed)
    smart00046
    Location:436 → 532
    DAGKc; Diacylglycerol kinase catalytic domain (presumed)
    cd00029
    Location:272 → 321
    C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
    cd00051
    Location:179 → 248
    EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
    pfam13499
    Location:180 → 244
    EF-hand_7; EF-hand domain pair
    pfam14513
    Location:5 → 175
    DAG_kinase_N; Diacylglycerol kinase N-terminus

  2. NM_001080745.2 → NP_001074214.1  diacylglycerol kinase gamma isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 3) that is shorter than isoform 1.
    Source sequence(s)
    AC112649, AC117414, BC089411, BC112363, BM669549
    Consensus CDS
    CCDS43181.1
    UniProtKB/TrEMBL
    Q2M589
    Related
    ENSP00000371599.4, ENST00000382164.8
    Conserved Domains (6) summary
    smart00045
    Location:539 → 713
    DAGKa; Diacylglycerol kinase accessory domain (presumed)
    smart00046
    Location:397 → 518
    DAGKc; Diacylglycerol kinase catalytic domain (presumed)
    cd00029
    Location:272 → 321
    C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
    cd00051
    Location:179 → 248
    EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
    pfam13499
    Location:180 → 244
    EF-hand_7; EF-hand domain pair
    pfam14513
    Location:5 → 175
    DAG_kinase_N; Diacylglycerol kinase N-terminus

  3. NM_001346.3 → NP_001337.2  diacylglycerol kinase gamma isoform 1

    See identical proteins and their annotated locations for NP_001337.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AC112649, AC117414, BC089411, BM669549, D26135
    Consensus CDS
    CCDS3274.1
    UniProtKB/Swiss-Prot
    B2RAH4, P49619, Q2M1H4, Q5FWG1
    UniProtKB/TrEMBL
    Q2M589
    Related
    ENSP00000265022.3, ENST00000265022.8
    Conserved Domains (7) summary
    smart00109
    Location:336 → 377
    C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)
    smart00045
    Location:578 → 752
    DAGKa; Diacylglycerol kinase accessory domain (presumed)
    smart00046
    Location:436 → 557
    DAGKc; Diacylglycerol kinase catalytic domain (presumed)
    cd00029
    Location:272 → 321
    C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
    cd00051
    Location:179 → 248
    EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
    pfam13499
    Location:180 → 244
    EF-hand_7; EF-hand domain pair
    pfam14513
    Location:5 → 175
    DAG_kinase_N; Diacylglycerol kinase N-terminus

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000003.12 Reference GRCh38.p14 Primary Assembly

    Range
    186147201..186362234 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060927.1 Alternate T2T-CHM13v2.0

    Range
    188962845..189177970 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P49619.3 GenPept UniProtKB/Swiss-Prot:P49619

Gene LinkOut

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