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G3BP1 G3BP stress granule assembly factor 1 [ Homo sapiens (human) ]

Gene ID: 10146, updated on 7-Apr-2024

Summary

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Official Symbol
G3BP1provided by HGNC
Official Full Name
G3BP stress granule assembly factor 1provided by HGNC
Primary source
HGNC:HGNC:30292
See related
Ensembl:ENSG00000145907 MIM:608431; AllianceGenome:HGNC:30292
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
G3BP; HDH-VIII
Summary
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in lymph node (RPKM 18.3), appendix (RPKM 16.8) and 25 other tissues See more
Orthologs
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Genomic context

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Location:
5q33.1
Exon count:
12
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 5 NC_000005.10 (151771954..151812785)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 5 NC_060929.1 (152310934..152351769)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 5 NC_000005.9 (151151515..151192346)

Chromosome 5 - NC_000005.10Genomic Context describing neighboring genes Neighboring gene ATAC-STARR-seq lymphoblastoid active region 23467 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 16527 Neighboring gene antioxidant 1 copper chaperone Neighboring gene ATOX1 antisense RNA 1 Neighboring gene uncharacterized LOC100652758 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr5:151149602-151150323 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 23468 Neighboring gene Sharpr-MPRA regulatory region 5918 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 16528 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 16529 Neighboring gene ribosomal protein lateral stalk subunit P1 pseudogene 6 Neighboring gene Sharpr-MPRA regulatory region 2380 Neighboring gene glycine receptor alpha 1 Neighboring gene glutamate-ammonia ligase pseudogene 1 Neighboring gene tRNA-Gln (anticodon CTG) 13-1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. G3BP1-dependent mechanism suppressing protein aggregation in Huntington's models and its demise upon stress granule assembly. Gutiérrez-Garcia R, et al. Hum Mol Genet, 2023 May 5. PMID 36611004, Free PMC Article
  2. The roles of G3BP1 in human diseases (review). Ge Y, et al. Gene, 2022 May 5. PMID 35176431
  3. G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities. He X, et al. Nucleic Acids Res, 2021 Nov 8. PMID 34614161, Free PMC Article
  4. Resveratrol and related stilbene derivatives induce stress granules with distinct clearance kinetics. Amen T, et al. Mol Biol Cell, 2021 Nov 1. PMID 34432484, Free PMC Article
  5. G3BP1 may serve as a potential biomarker of proliferation, apoptosis, and prognosis in oral squamous cell carcinoma. Hu X, et al. J Oral Pathol Med, 2021 Nov. PMID 33987877

See all (355) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. DHX38 enhances proliferation, metastasis, and EMT progression in NSCLC through the G3BP1-mediated MAPK pathway.
    Title: DHX38 enhances proliferation, metastasis, and EMT progression in NSCLC through the G3BP1-mediated MAPK pathway.
  2. Endometrial cancer (EC) derived G3BP1 overexpression and mutant promote EC tumorigenesis and metastasis via SPOP/ERalpha axis.
    Title: Endometrial cancer (EC) derived G3BP1 overexpression and mutant promote EC tumorigenesis and metastasis via SPOP/ERα axis.
  3. Phase-separated nucleocapsid protein of SARS-CoV-2 suppresses cGAS-DNA recognition by disrupting cGAS-G3BP1 complex.
    Title: Phase-separated nucleocapsid protein of SARS-CoV-2 suppresses cGAS-DNA recognition by disrupting cGAS-G3BP1 complex.
  4. TRIM25-mediated ubiquitination of G3BP1 regulates the proliferation and migration of human neuroblastoma cells.
    Title: TRIM25-mediated ubiquitination of G3BP1 regulates the proliferation and migration of human neuroblastoma cells.
  5. The DUSP domain of pseudophosphatase MK-STYX interacts with G3BP1 to decrease stress granules.
    Title: The DUSP domain of pseudophosphatase MK-STYX interacts with G3BP1 to decrease stress granules.
  6. Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules.
    Title: Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules.
  7. The stress granule protein G3BP1 alleviates spinocerebellar ataxia-associated deficits.
    Title: The stress granule protein G3BP1 alleviates spinocerebellar ataxia-associated deficits.
  8. G3BP1-dependent mechanism suppressing protein aggregation in Huntington's models and its demise upon stress granule assembly.
    Title: G3BP1-dependent mechanism suppressing protein aggregation in Huntington's models and its demise upon stress granule assembly.
  9. Caprin-1 binding to the critical stress granule protein G3BP1 is influenced by pH.
    Title: Caprin-1 binding to the critical stress granule protein G3BP1 is influenced by pH.
  10. The force-dependent filamin A-G3BP1 interaction regulates phase-separated stress granule formation.
    Title: The force-dependent filamin A-G3BP1 interaction regulates phase-separated stress granule formation.
Submit: New GeneRIF Correction See all GeneRIFs (109)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
A genome-wide association meta-analysis of preschool internalizing problems.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 is identified to have a physical interaction with GTPase activating protein (SH3 domain) binding protein 1 (G3BP1) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
Pr55(Gag) gag HIV-1 Gag inhibits G3BP1 localization in U2OS cells; inhibiting the formation of stress granules PubMed
gag HIV-1 Gag co-localizes and interacts with G3BP1 and TIAR to modulate stress granule assembly during HIV-1 infection PubMed
gag HIV-1 Gag has the ability to interfere with HIV-2 Gag induced assembly of stress granules with the protein marker G3BP1 PubMed
gag Overexpression of G3BP1 inhibits the interaction between HIV-1 Gag and eEF2 in cells, suggesting that G3BP1 competes with eEF2 binding to Gag PubMed
Tat tat GTPase activating protein (SH3 domain) binding protein 1 (G3BP1) is identified to interact with HIV-1 Tat mutant Nullbasic in HeLa cells by LC MS/MS PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC111040

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA/RNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA binding HDA PubMed 
enables RNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables mRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables molecular condensate scaffold activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ribosomal small subunit binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA duplex unwinding IEA
Inferred from Electronic Annotation
more info
  
involved_in Ras protein signal transduction TAS
Traceable Author Statement
more info
 PubMed 
involved_in defense response to virus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in innate immune response IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of canonical Wnt signaling pathway IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of stress granule assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of type I interferon production IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of type I interferon production IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in stress granule assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in stress granule assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasmic stress granule IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasmic stress granule IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasmic stress granule ISS
Inferred from Sequence or Structural Similarity
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
  
located_in focal adhesion HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in perikaryon IEA
Inferred from Electronic Annotation
more info
  
part_of ribonucleoprotein complex IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
ras GTPase-activating protein-binding protein 1
Names
ATP-dependent DNA helicase VIII
DNA helicase VIII
G3BP-1
GAP SH3 domain-binding protein 1
GAP binding protein
GTPase activating protein (SH3 domain) binding protein 1
Ras-GTPase-activating protein SH3-domain-binding protein
RasGAP-associated endoribonuclease G3BP
NP_005745.1
NP_938405.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_005754.3 → NP_005745.1  ras GTPase-activating protein-binding protein 1

    See identical proteins and their annotated locations for NP_005745.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform.
    Source sequence(s)
    AC091982, AK130003, BU517281, BX647869, BX648012
    Consensus CDS
    CCDS4319.1
    UniProtKB/Swiss-Prot
    Q13283, Q5HYE9
    UniProtKB/TrEMBL
    Q32P45, Q5U0Q1, Q6FI03
    Related
    ENSP00000348578.3, ENST00000356245.8
    Conserved Domains (3) summary
    pfam06390
    Location:212 → 320
    NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
    cd00780
    Location:7 → 135
    NTF2; Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many ...
    cd12463
    Location:337 → 416
    RRM_G3BP1; RNA recognition motif found in ras GTPase-activating protein-binding protein 1 (G3BP1) and similar proteins

  2. NM_198395.2 → NP_938405.1  ras GTPase-activating protein-binding protein 1

    See identical proteins and their annotated locations for NP_938405.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform.
    Source sequence(s)
    AC091982, AK130003, BX647869, BX648012
    Consensus CDS
    CCDS4319.1
    UniProtKB/Swiss-Prot
    Q13283, Q5HYE9
    UniProtKB/TrEMBL
    Q32P45, Q5U0Q1, Q6FI03
    Related
    ENSP00000377681.3, ENST00000394123.7
    Conserved Domains (3) summary
    pfam06390
    Location:212 → 320
    NESP55; Neuroendocrine-specific golgi protein P55 (NESP55)
    cd00780
    Location:7 → 135
    NTF2; Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many ...
    cd12463
    Location:337 → 416
    RRM_G3BP1; RNA recognition motif found in ras GTPase-activating protein-binding protein 1 (G3BP1) and similar proteins

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000005.10 Reference GRCh38.p14 Primary Assembly

    Range
    151771954..151812785
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060929.1 Alternate T2T-CHM13v2.0

    Range
    152310934..152351769
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q13283.1 GenPept UniProtKB/Swiss-Prot:Q13283

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