Schneider fetal brain 00004

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Result Filters

Organism: Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo

Attributes

sex	male
tissue	frontal lobe
development stage	5 months post-conception
lab host	DH10B
tissue	brain
vector	pBluescript SK (Stratagene)
v_type	plasmid (ampicillin resistant)
re_1	SstI
re_2	XhoI

Description: Double-stranded cDNA was prepared from human fetal brain tissue. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-GAGAGAGAGAAGAGCTCAAGGATCCTTAATTAAATTAATCCCCCCCCCCCCC-3' and 3' adaptor sequence: 5'-GAGAGAGAGACTCGAGTTTTTTTTTTTTTTTTTT-3'. The library was size-selected for >0.5 kb inserts and has an average insert size estimated at 1.2 kb. This library was constructed using the CAP-trapper method for full-length enrichment and has not undergone amplification. Library was constructed by Dr. Claudio Schneider (LNCIB-Area Science Park, Trieste, Italy).