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Conserved domains on  [gi|1034595304|ref|XP_016878945|]
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centromere protein N isoform X3 [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CENP-N super family cl08436
Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a ...
 5-165 2.59e-49

Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a component of the central kinetochore which mediates the attachment of the centromere to the mitotic spindle. CENP-N is one of the components that assembles onto the CENP-A-nucleosome-associated (NAC) centromere. The centromere, which is the basic element of chromosome inheritance, is epigenetically determined in mammals. CENP-A, the centromere-specific histone H3 variant, assembles an array of nucleosomes and it is this that seems to be the prime candidate for specifying centromere identity. CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of CENP-M, CENP-N and CENP-T, CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Additionally, there are seven other subunits which make up the CENP-A-nucleosome distal (CAD) centromere, CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S, also assembling on the CENP-A NAC.


The actual alignment was detected with superfamily member pfam05238:

Pssm-ID: 398764  Cd Length: 403  Bit Score: 164.92  E-value: 2.59e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034595304   5 VAEFIKRTILKIPMNELTTILKAW------------------DFLSENQLQTVNF------RQRKESVVQHLIHLCEEKR 60
Cdd:pfam05238   2 TTPVLRRILLRLPRSSLLDLLQKWldkrntqpiddgddededGFLPERTLQTLRElyqelqKGPKREVIDRILEGDWPHG 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034595304  61 ASISDAALLDIIYMQFHQHQKVWEVFQMSKGPG-EDVDLFDMKQFKNSFKKILQRALKNVTVSFRETEENAVWIRI-AWG 138
Cdd:pfam05238  82 LNLYQLAQLDIQYLVDHPNSLRWTASQLSDSDGkEVIPRLDPSTFLSNLQEQLSPLYKAHYYLFRHPELPLIIIRIqLFD 161

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 1034595304 139 TQYTKPNQ--------YKPTYVVYYSQTPYAFTSS 165
Cdd:pfam05238 162 SPYSTPNQapeplldsSKPFYVAFPDNSPYVFISS 196
 
Name Accession Description Interval E-value
CENP-N pfam05238
Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a ...
 5-165 2.59e-49

Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a component of the central kinetochore which mediates the attachment of the centromere to the mitotic spindle. CENP-N is one of the components that assembles onto the CENP-A-nucleosome-associated (NAC) centromere. The centromere, which is the basic element of chromosome inheritance, is epigenetically determined in mammals. CENP-A, the centromere-specific histone H3 variant, assembles an array of nucleosomes and it is this that seems to be the prime candidate for specifying centromere identity. CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of CENP-M, CENP-N and CENP-T, CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Additionally, there are seven other subunits which make up the CENP-A-nucleosome distal (CAD) centromere, CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S, also assembling on the CENP-A NAC.


Pssm-ID: 398764  Cd Length: 403  Bit Score: 164.92  E-value: 2.59e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034595304   5 VAEFIKRTILKIPMNELTTILKAW------------------DFLSENQLQTVNF------RQRKESVVQHLIHLCEEKR 60
Cdd:pfam05238   2 TTPVLRRILLRLPRSSLLDLLQKWldkrntqpiddgddededGFLPERTLQTLRElyqelqKGPKREVIDRILEGDWPHG 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034595304  61 ASISDAALLDIIYMQFHQHQKVWEVFQMSKGPG-EDVDLFDMKQFKNSFKKILQRALKNVTVSFRETEENAVWIRI-AWG 138
Cdd:pfam05238  82 LNLYQLAQLDIQYLVDHPNSLRWTASQLSDSDGkEVIPRLDPSTFLSNLQEQLSPLYKAHYYLFRHPELPLIIIRIqLFD 161

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 1034595304 139 TQYTKPNQ--------YKPTYVVYYSQTPYAFTSS 165
Cdd:pfam05238 162 SPYSTPNQapeplldsSKPFYVAFPDNSPYVFISS 196
 
Name Accession Description Interval E-value
CENP-N pfam05238
Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a ...
 5-165 2.59e-49

Kinetochore protein CHL4 like; CHL4 is a protein involved in chromosome segregation. It is a component of the central kinetochore which mediates the attachment of the centromere to the mitotic spindle. CENP-N is one of the components that assembles onto the CENP-A-nucleosome-associated (NAC) centromere. The centromere, which is the basic element of chromosome inheritance, is epigenetically determined in mammals. CENP-A, the centromere-specific histone H3 variant, assembles an array of nucleosomes and it is this that seems to be the prime candidate for specifying centromere identity. CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of CENP-M, CENP-N and CENP-T, CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Additionally, there are seven other subunits which make up the CENP-A-nucleosome distal (CAD) centromere, CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S, also assembling on the CENP-A NAC.


Pssm-ID: 398764  Cd Length: 403  Bit Score: 164.92  E-value: 2.59e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034595304   5 VAEFIKRTILKIPMNELTTILKAW------------------DFLSENQLQTVNF------RQRKESVVQHLIHLCEEKR 60
Cdd:pfam05238   2 TTPVLRRILLRLPRSSLLDLLQKWldkrntqpiddgddededGFLPERTLQTLRElyqelqKGPKREVIDRILEGDWPHG 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1034595304  61 ASISDAALLDIIYMQFHQHQKVWEVFQMSKGPG-EDVDLFDMKQFKNSFKKILQRALKNVTVSFRETEENAVWIRI-AWG 138
Cdd:pfam05238  82 LNLYQLAQLDIQYLVDHPNSLRWTASQLSDSDGkEVIPRLDPSTFLSNLQEQLSPLYKAHYYLFRHPELPLIIIRIqLFD 161

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 1034595304 139 TQYTKPNQ--------YKPTYVVYYSQTPYAFTSS 165
Cdd:pfam05238 162 SPYSTPNQapeplldsSKPFYVAFPDNSPYVFISS 196
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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