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Conserved domains on  [gi|255304948|ref|NP_001157285|]
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twinkle mtDNA helicase isoform C [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Twinkle_C cd01122
C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid ...
 1-175 1.36e-117

C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid localization, also known as C10orf2, PEO1, SCA8, ATXN8, IOSCA, PEOA3 or SANDO) is a homohexameric DNA helicases which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. Mutations in the human gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. This group also contains viral GP4-like and related bacterial helicases.


:

Pssm-ID: 410867  Cd Length: 266  Bit Score: 335.75  E-value: 1.36e-117
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948   1 MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAA 80
Cdd:cd01122   90 MLTQFAGKNLEDNLREFDEWADKFELLPMYFMKFHGSTDIDEVLDAMEHAVYVYDIQHIVIDNLQFMMGTQASGSDRFEL 169

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948  81 QDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDV 160
Cdd:cd01122  170 QDLIIGKFRRFATNNNVHITLVIHPRKEDDDNELTTSSIFGSAKATQEADNVLILQDKRLISGEGKKFLQIKKNRFDGDL 249

                        170
                 ....*....|....*
gi 255304948 161 GVFPLEFNKNSLTFS 175
Cdd:cd01122  250 GVIPLEFNKNSLTYS 264
 
Name Accession Description Interval E-value
Twinkle_C cd01122
C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid ...
 1-175 1.36e-117

C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid localization, also known as C10orf2, PEO1, SCA8, ATXN8, IOSCA, PEOA3 or SANDO) is a homohexameric DNA helicases which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. Mutations in the human gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. This group also contains viral GP4-like and related bacterial helicases.


Pssm-ID: 410867  Cd Length: 266  Bit Score: 335.75  E-value: 1.36e-117
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948   1 MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAA 80
Cdd:cd01122   90 MLTQFAGKNLEDNLREFDEWADKFELLPMYFMKFHGSTDIDEVLDAMEHAVYVYDIQHIVIDNLQFMMGTQASGSDRFEL 169

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948  81 QDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDV 160
Cdd:cd01122  170 QDLIIGKFRRFATNNNVHITLVIHPRKEDDDNELTTSSIFGSAKATQEADNVLILQDKRLISGEGKKFLQIKKNRFDGDL 249

                        170
                 ....*....|....*
gi 255304948 161 GVFPLEFNKNSLTFS 175
Cdd:cd01122  250 GVIPLEFNKNSLTYS 264
AAA_25 pfam13481
AAA domain; This AAA domain is found in a wide variety of presumed DNA repair proteins.
 9-110 1.46e-11

AAA domain; This AAA domain is found in a wide variety of presumed DNA repair proteins.


Pssm-ID: 463892 [Multi-domain]  Cd Length: 193  Bit Score: 61.24  E-value: 1.46e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948    9 RLEDQLDKYDHWA-----DRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAqdy 83
Cdd:pfam13481  90 RLRAAGADLDLPArllflSLVESLPLFFLDRGGPLLDADVDALEAALEEVEDPDLVVIDPLARALGGDENSNSDVGR--- 166

                          90       100
                  ....*....|....*....|....*..
gi 255304948   84 IIGVFRKFATDNNCHVTLVIHPRKEDD 110
Cdd:pfam13481 167 LVKALDRLARRTGATVLLVHHVGKDGA 193
RepA COG3598
RecA-family ATPase [Replication, recombination and repair];
9-156 1.12e-06

RecA-family ATPase [Replication, recombination and repair];


Pssm-ID: 442817 [Multi-domain]  Cd Length: 313  Bit Score: 48.36  E-value: 1.12e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948   9 RLEDQLDKYDHWADRFEDlPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAqdyIIGVF 88
Cdd:COG3598   68 RLKALGADLGLPFADLDG-RLRLLSLAGDLDDTDDLEALERAIEEEGPDLVVIDPLARVFGGDENDAEEMRA---FLNPL 143

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 255304948  89 RKFATDNNCHVTLVIHPRKeDDDKELQTASIFGSAKASQEADNVLILQDrklVTGPGKRYLQVSKNRF 156
Cdd:COG3598  144 DRLAERTGAAVLLVHHTGK-GGAGKDSGDRARGSSALRGAARSVLVLSR---EKGEDLRVLTRAKSNY 207
 
Name Accession Description Interval E-value
Twinkle_C cd01122
C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid ...
 1-175 1.36e-117

C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid localization, also known as C10orf2, PEO1, SCA8, ATXN8, IOSCA, PEOA3 or SANDO) is a homohexameric DNA helicases which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. Mutations in the human gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. This group also contains viral GP4-like and related bacterial helicases.


Pssm-ID: 410867  Cd Length: 266  Bit Score: 335.75  E-value: 1.36e-117
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948   1 MLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAA 80
Cdd:cd01122   90 MLTQFAGKNLEDNLREFDEWADKFELLPMYFMKFHGSTDIDEVLDAMEHAVYVYDIQHIVIDNLQFMMGTQASGSDRFEL 169

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948  81 QDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDV 160
Cdd:cd01122  170 QDLIIGKFRRFATNNNVHITLVIHPRKEDDDNELTTSSIFGSAKATQEADNVLILQDKRLISGEGKKFLQIKKNRFDGDL 249

                        170
                 ....*....|....*
gi 255304948 161 GVFPLEFNKNSLTFS 175
Cdd:cd01122  250 GVIPLEFNKNSLTYS 264
RecA-like_superfamily cd01120
RecA-like_NTPases; RecA-like NTPases. This superfamily includes the NTP binding domain of F1 ...
 44-122 1.08e-11

RecA-like_NTPases; RecA-like NTPases. This superfamily includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases. This group also includes bacterial conjugation proteins and related DNA transfer proteins involved in type II and type IV secretion.


Pssm-ID: 410865 [Multi-domain]  Cd Length: 119  Bit Score: 59.82  E-value: 1.08e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948  44 IDTMQHAVYV----YDICHVIIDNLQFMMGHEQlsTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASI 119
Cdd:cd01120   35 LDTILEAIEDlieeKKLDIIIIDSLSSLARASQ--GDRSSELLEDLAKLLRAARNTGITVIATIHSDKFDIDRGGSSNDE 112


                 ...
gi 255304948 120 FGS 122
Cdd:cd01120  113 RLL 115
AAA_25 pfam13481
AAA domain; This AAA domain is found in a wide variety of presumed DNA repair proteins.
 9-110 1.46e-11

AAA domain; This AAA domain is found in a wide variety of presumed DNA repair proteins.


Pssm-ID: 463892 [Multi-domain]  Cd Length: 193  Bit Score: 61.24  E-value: 1.46e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948    9 RLEDQLDKYDHWA-----DRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAqdy 83
Cdd:pfam13481  90 RLRAAGADLDLPArllflSLVESLPLFFLDRGGPLLDADVDALEAALEEVEDPDLVVIDPLARALGGDENSNSDVGR--- 166

                          90       100
                  ....*....|....*....|....*..
gi 255304948   84 IIGVFRKFATDNNCHVTLVIHPRKEDD 110
Cdd:pfam13481 167 LVKALDRLARRTGATVLLVHHVGKDGA 193
RecA-like_Gp4D_helicase cd19483
RecA-like domain of Escherichia coli bacteriophage T7 Gp4D helicase; This family includes the ...
 6-169 9.63e-10

RecA-like domain of Escherichia coli bacteriophage T7 Gp4D helicase; This family includes the RecA-like domain of the Gp4D fragment of the Gene4 helicase-primase (Gp4) from bacteriophage T7. Gp4D (residues 241-566) is the minimal fragment of the Gp4 that forms hexameric rings, it contains the helicase domain and the linker connecting the helicase and primase domains. Helicases are ring-shaped oligomeric enzymes that unwind DNA at the replication fork; they couple NTP hydrolysis to the unwinding of nucleic acid duplexes into their component strands. This family belongs to the RecA-like NTPase superfamily which includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases. The RecA-like NTPase family also includes bacterial conjugation proteins and related DNA transfer proteins involved in type II and type IV secretion.


Pssm-ID: 410891 [Multi-domain]  Cd Length: 231  Bit Score: 56.81  E-value: 9.63e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948   6 AEGRLEDQLDKYDHWADRFEDLP-LYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQfMMGHEQLSTDRIAAQDYI 84
Cdd:cd19483   58 ELPRDDITEEEEDDAFDNELGSGrFFLYDHFGSLDWDNLKEKIRYMVKVLGCKVIVLDHLT-ILVSGLDSSDERKELDEI 136

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948  85 IGVFRKFATDNNCHVTLVIHPRKEDDDK------ELQTASIFGSAKASQEADNVLILQDRKLVTGPGKR---YLQVSKNR 155
Cdd:cd19483  137 MTELAALVKELGVTIILVSHLRRPGGGKgheeggEVSESDLRGSSAIAQLSDYVIGLERNKQADDPVERnttRVRVLKNR 216

                        170
                 ....*....|....*
gi 255304948 156 FDGDVGVFP-LEFNK 169
Cdd:cd19483  217 FTGETGIAGtLYYDE 231
RepA COG3598
RecA-family ATPase [Replication, recombination and repair];
9-156 1.12e-06

RecA-family ATPase [Replication, recombination and repair];


Pssm-ID: 442817 [Multi-domain]  Cd Length: 313  Bit Score: 48.36  E-value: 1.12e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 255304948   9 RLEDQLDKYDHWADRFEDlPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAqdyIIGVF 88
Cdd:COG3598   68 RLKALGADLGLPFADLDG-RLRLLSLAGDLDDTDDLEALERAIEEEGPDLVVIDPLARVFGGDENDAEEMRA---FLNPL 143

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 255304948  89 RKFATDNNCHVTLVIHPRKeDDDKELQTASIFGSAKASQEADNVLILQDrklVTGPGKRYLQVSKNRF 156
Cdd:COG3598  144 DRLAERTGAAVLLVHHTGK-GGAGKDSGDRARGSSALRGAARSVLVLSR---EKGEDLRVLTRAKSNY 207
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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