1LF8,1MHQ,1PY1


Conserved Protein Domain Family
VHS_GGA
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cd03567: VHS_GGA (this model, PSSM-Id:239624 is obsolete and has been replaced by 340769)
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VHS domain family, GGA subfamily; GGA (Golgi-localized, Gamma-ear-containing, Arf-binding) comprise a subfamily of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (gamma-adaptin ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the trans-Golgi network and the endosomal system.
Links
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Statistics
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PSSM-Id: 239624
Aligned: 12 rows
Threshold Bit Score: 216.078
Created: 1-Mar-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
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acidic-cluste..putative
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:acidic-cluster-dileucine motif interaction site
Evidence:
  • Structure:1LF8_A; VHS domain of human GGA3 bound to mannose 6-phosphate receptor fragment; contacts at 3.5A
    - View structure with Cn3D
  • Structure:1PY1_D; VHS domain of human GGA1 bound to beta-secretase phosphoserine peptide; contacts at 3.5A
    - View structure with Cn3D
  • Comment:Recognition site of acidic-cluster-dilleucine signal
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                    #
1LF8_A     13 SLESWLNKATNPSNr-QEDWEYIIGFCDQINkELEGPQIAVRLLAHKIQSPQEWEALQALTVLEACMKNCGRRFHNEVGK 91  Homo sapiens
1MHQ_B      1 SLELWLNKATDPSMs-EQDWSAIQNFCEQVNtDPNGPTHAPWLLAHKIQSPQEKEALYALTVLEMCMNHCGEKFHSEVAK 79  human
1PY1_D     10 TLEARINRATNPLNk-ELDWASINGFCEQLNeDFEGPPLATRLLAHKIQSPQEWEAIQALTVLETCMKSCGKRFHDEVGK 88  human
NP_572571   7 IMEEMLERATNPAQ--KIDELGVQMFCIVVKsNAQLVHKAQEMIVAKVRSTNVTEATRAISLLEECMTQCGDDFQDEASK 84  fruit fly
AAH73000    5 SLEVQINRATNPLNk-EQDWDGVQAFCDQLNqELEGPQLATRLLAHKIQSPQEWEAMQALTVLEACMKNCGKRFHSEVGK 83  African clawed frog
CAH65038    8 SLESWLNKATNPSNr-QEDWEYIIGFCDQINkELEGPQIAVRLLAHKIQSPQEWEAVQALTVLEACMKNCGRRFHNEVGK 86  chicken
XP_393222   7 SLEALIQRVTNPQNq-KPDIAAIEAFCVMLTkESEGVQIGTKLLALHIQSSNETEALQALALLDTCMRRCGPSFHAEVGK 85  honey bee
XP_698317   8 SLESWLNKATNPSNr-QEEWEYIIGFCDQINkELEGPQISVRLLAHKIQSPQEWESLQALTVLEACMKNCGGRFHNEVGK 86  zebrafish
XP_789390   9 TLETLLNKATNPSNr-DDDWEYIMNFCDRVNsELEGALLSCRLLGHKIQSPQEREALQALTVIEACVKNCGELFHRELGK 87  purple urchin
AAX28464    3 ELEKLLSKATNPISd-QYNSETISEIAKLIDtQPNGPIFTLRLLAHKIKSPHEKEALNALSLLESLSKRCAPTFISELGK 81  Schistosoma japonicum
XP_972937   8 SLEALLLKATNSQNq-NIDTAAVEAFCALVNkEKDGAHIGVKVIANRLPSGNEKELLQTLNILDTCMSKCGTAFQSEVGK 86  red flour beetle
CAA92026    8 PIDYWVCRATDRFIgdEERIKALDFIIESIYkQPASALLAVDLFSHKLSSPSQEEAMLTIRALDYLVRNGGEKVHERCGR 87  nematode

Feature 1     ##  #        ##                             #      #   #     
1LF8_A     92 FRFLNELIKVVSPKYLGDRVSEKVKTKVIELLYSWTMALP-EEAKIKDAYHMLKRQGIVQS 151 Homo sapiens
1MHQ_B     80 FRFLNELIKVLSPKYLGSWATGKVKGRVIEILFSWTVWFP-EDIKIRDAYQMLKKQGIIKQ 139 human
1PY1_D     89 FRFLNELIKVVSPKYLGSRTSEKVKNKILELLYSWTVGLP-EEVKIAEAYQMLKKQGIVKS 148 human
NP_572571  85 FRFLNELIRLVSKKYKGAETPHEVKQRIMECLLLWTTEFP-QRQKIRDAYDMLRKEGDIEH 144 fruit fly
AAH73000   84 FRFLNELIKVVSPKYLGSRSPEKVKLKILELLYSWTLGLP-SEVKISEAYQMLKKQGIVKE 143 African clawed frog
CAH65038   87 FRFLNELIKVVSPKYLGDRVSEKVKTKVIELLYSWTVALP-EEYKIKDAYYMLKRQGIVMF 146 chicken
XP_393222  86 FRFLNEMIRLVSPKYLGGKTPAIVRQKVLQLLNMWTKEYP-KELKIKEAYEMLKKQGVIED 145 honey bee
XP_698317  87 FRFLNELIKLVSPKYLGDRVSERVKTKVIELLYNCSVALP-DEAKIAEAYHMLKKQGCFMT 146 zebrafish
XP_789390  88 FRFLNEMIKLISPKYLGNKTTEKVKKKTIELMYSWQKGLP-HEGKIVEAYDMLKKQATRRA 147 purple urchin
AAX28464   82 FKFLNELIKVLSPKYLGDQTSSSVKDKCAQLLHNWQRDFSqTEPKFAEAYNMLVREGIITS 142 Schistosoma japonicum
XP_972937  87 FRFLNEMIKLVSPKYLGSQTPLVVKQKVLQLMYIWTLDYP-KETKIKEAYDMLRKQGVIKE 146 red flour beetle
CAA92026   88 YKFLNELVRLIAPKYNGKLTSDALKTEIIKLLFIWQLSIK-HIPKYKQVYESLKLSKIITD 147 nematode

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