Dcaf1 DDB1 and CUL4 associated factor 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dcaf1provided by MGI
Official Full Name: DDB1 and CUL4 associated factor 1provided by MGI
Primary source: MGI:MGI:2445220
See related: Ensembl:ENSMUSG00000040325 AllianceGenome:MGI:2445220
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Vprbp; mKIAA0800; B930007L02Rik
Summary: Predicted to enable estrogen receptor binding activity and histone kinase activity (H2A-T120 specific). Involved in B cell differentiation and V(D)J recombination. Predicted to be located in fibrillar center and nucleoplasm. Predicted to be part of COP9 signalosome and Cul4-RING E3 ubiquitin ligase complex. Predicted to be active in nucleus. Orthologous to human DCAF1 (DDB1 and CUL4 associated factor 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in testis adult (RPKM 24.0), CNS E11.5 (RPKM 10.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 F1; 9 57.98 cM

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (106691354..106758191)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (106814169..106880992)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DCAF1 regulates Treg senescence via the ROS axis during immunological aging.
Title: DCAF1 regulates Treg senescence via the ROS axis during immunological aging.
VprBP mitigates TGF-beta and Activin signaling by promoting Smurf1-mediated type I receptor degradation.
Title: VprBP mitigates TGF-β and Activin signaling by promoting Smurf1-mediated type I receptor degradation.
The basis for RAG1 turnover and provide evidence that the CRL4(VprBP(DCAF1)) complex functions to maintain physiological levels of V(D)J recombination.
Title: VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by Mediating RAG1 Degradation.
DCAF1 is required for T-cell expansion and function during anti-viral and autoimmune responses.
Title: DCAF1 controls T-cell function via p53-dependent and -independent mechanisms.
VprBP Is Required for Efficient Editing and Selection of Igkappa+ B Cells, but Is Dispensable for Iglambda+ and Marginal Zone B Cell Maturation and Selection
Title: VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection.
CRL4(VPRBP) ubiquitin ligase is a guardian of female reproductive life in germ cells and a maternal reprogramming factor after fertilization.
Title: CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.
multi-subunit cullin RING E3 ligase complex VprBP/DDB1/Cul4A/Roc1 associates with full-length RAG1 through VprBP
Title: VprBP binds full-length RAG1 and is required for B-cell development and V(D)J recombination fidelity.
Data show that human immunodeficiency virus type 1 Vpr-binding protein VprBP binds stoichiometrically with DDB1 through its WD40 domain and through DDB1 to CUL4A, subunits of the COP9/signalsome.
Title: Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (2) 1 citation
Transgenic (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH137304 (e-PCR)
- UniSTS:219392

AI447437 (e-PCR)
- UniSTS:160789

C79467 (e-PCR)
- UniSTS:208441

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables histone H2AT120 kinase activity	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nuclear estrogen receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables nuclear estrogen receptor binding	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-like ligase-substrate adaptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in B cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in V(D)J recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell competition in a multicellular organism	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in post-translational protein modification	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of COP9 signalosome	ISO Inferred from Sequence Orthology more info
part_of Cul4-RING E3 ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Cul4-RING E3 ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in fibrillar center	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: DDB1- and CUL4-associated factor 1

Names: Vpr (HIV-1) binding protein; protein VPRBP; serine/threonine-protein kinase VPRBP

NP_001015507.1

EC 2.7.11.1

NP_001360859.1

EC 2.7.11.1

NP_001360860.1

EC 2.7.11.1

XP_006511816.1

EC 2.7.11.1

XP_006511817.1

EC 2.7.11.1

XP_036010962.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001015507.3 → NP_001015507.1 DDB1- and CUL4-associated factor 1 isoform 3

See identical proteins and their annotated locations for NP_001015507.1

Status: VALIDATED

Source sequence(s)

AC147636

Consensus CDS

CCDS23485.1

UniProtKB/Swiss-Prot

Q80TR8

Related

ENSMUSP00000123865.2, ENSMUST00000159645.8

Conserved Domains (2) summary

sd00039
Location:1096 → 1132

7WD40; WD40 repeat [structural motif]

cl29593
Location:1092 → 1310

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_001373930.1 → NP_001360859.1 DDB1- and CUL4-associated factor 1 isoform 1

Status: VALIDATED

Source sequence(s)

AC147636

Consensus CDS

CCDS90657.1

UniProtKB/Swiss-Prot

Q3UXD5, Q6P1E2, Q80TR8, Q8CDY3

Related

ENSMUSP00000060025.9, ENSMUST00000055009.15

Conserved Domains (2) summary

sd00039
Location:1096 → 1132

7WD40; WD40 repeat [structural motif]

cl29593
Location:1092 → 1310

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_001373931.1 → NP_001360860.1 DDB1- and CUL4-associated factor 1 isoform 2

Status: VALIDATED

Source sequence(s)

AC147636

Conserved Domains (2) summary

sd00039
Location:1043 → 1079

7WD40; WD40 repeat [structural motif]

cl29593
Location:1039 → 1257

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

106691354..106758191

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006511753.4 → XP_006511816.1 DDB1- and CUL4-associated factor 1 isoform X1

Conserved Domains (2) summary

sd00039
Location:1106 → 1142

7WD40; WD40 repeat [structural motif]

cl29593
Location:1102 → 1320

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
XM_006511754.4 → XP_006511817.1 DDB1- and CUL4-associated factor 1 isoform X2

Conserved Domains (2) summary

sd00039
Location:1106 → 1142

7WD40; WD40 repeat [structural motif]

cl29593
Location:1102 → 1320

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
XM_036155069.1 → XP_036010962.1 DDB1- and CUL4-associated factor 1 isoform X3

Conserved Domains (2) summary

sd00039
Location:1096 → 1132

7WD40; WD40 repeat [structural motif]

cl29593
Location:1092 → 1310

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...