Srgap3 SLIT-ROBO Rho GTPase activating protein 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Srgap3provided by MGI
Official Full Name: SLIT-ROBO Rho GTPase activating protein 3provided by MGI
Primary source: MGI:MGI:2152938
See related: Ensembl:ENSMUSG00000030257 AllianceGenome:MGI:2152938
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Gbi; WRP; MEGAP; Arhgap14; mKIAA0411; D130026O08Rik
Summary: Predicted to enable GTPase activator activity. Predicted to be involved in negative regulation of cell migration. Predicted to be active in cytoplasm. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Used to study schizophrenia. Orthologous to human SRGAP3 (SLIT-ROBO Rho GTPase activating protein 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in frontal lobe adult (RPKM 27.6), CNS E18 (RPKM 23.8) and 12 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 E3; 6 52.54 cM

Exon count:: 24

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (112694932..112924466, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (112717971..112947505, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SrGAP3 plays a role in neurodevelopmental disorders.
Title: Morphological and behavioral characterization of adult mice deficient for SrGAP3.
This study found a more than 15-fold enlargement of the lateral ventricles of homozygous SrGAP3-deficient mice.
Title: SrGAP3 knockout mice display enlarged lateral ventricles and specific cilia disturbances of ependymal cells in the third ventricle.
SrGAP3 dynamically regulates neuronal cytoskeletal reorganization through inhibition of the Rho GTPase Rac1 and interaction with actin remodeling proteins.
Title: The cellular function of srGAP3 and its role in neuronal morphogenesis.
The inverse F-BAR domain protein srGAP2 acts through srGAP3 to modulate neuronal differentiation and neurite outgrowth of mouse neuroblastoma cells.
Title: The inverse F-BAR domain protein srGAP2 acts through srGAP3 to modulate neuronal differentiation and neurite outgrowth of mouse neuroblastoma cells.
Disruption of wave-associated Rac GTPase-activating protein (Wrp) leads to abnormal adult neural progenitor migration associated with hydrocephalus
Title: Disruption of wave-associated Rac GTPase-activating protein (Wrp) leads to abnormal adult neural progenitor migration associated with hydrocephalus.
These results show neurodevelopmental aberration in Srgap3(-/-) mice, with many of the observed phenotypes matching several schizophrenia-related intermediate phenotypes. Mutations of SRGAP3 may thus contribute to various neurodevelopmental disorders.
Title: Srgap3⁻/⁻ mice present a neurodevelopmental disorder with schizophrenia-related intermediate phenotypes.
Following sciatic nerve transection, srGAP3 in neurons is significantly increased in the ipsilateral relative to contralateral dorsal root ganglions, which peak at day 7 to day 14.
Title: Slit-Robo GTPase-activating proteins are differentially expressed in murine dorsal root ganglia: modulation by peripheral nerve injury.
srGAP3 is an inhibitor of actin dynamics and lamellipodia formation in primary fibroblasts. srGAP3 influences focal adhesion formation through the downregulation of Rac1.
Title: SrGAP3 interacts with lamellipodin at the cell membrane and regulates Rac-dependent cellular protrusions.
Data suggest a role for srGAP3 in the lateral positioning of post crossing axons within the ventrolateral funiculus.
Title: Evidence for a role of srGAP3 in the positioning of commissural axons within the ventrolateral funiculus of the mouse spinal cord.
srGAP3 could regulate neuronal differentiation in a Rac1-dependent manner.
Title: The mental retardation associated protein, srGAP3 negatively regulates VPA-induced neuronal differentiation of Neuro2A cells.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D6Mit328 (e-PCR), detects polymorphism
- UniSTS:130882

AI452337 (e-PCR)
- UniSTS:159556

Srgap3 (e-PCR), detects polymorphism
- UniSTS:507244

RH131868 (e-PCR)
- UniSTS:215151

30.MMHAP28FRB12.seq (e-PCR)
- UniSTS:123899

D6Oda19 (e-PCR), detects polymorphism
- UniSTS:515325

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of postsynapse assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: SLIT-ROBO Rho GTPase-activating protein 3

Names: SLIT-ROBO Rho GTPase activating protein 2; WAVE-associated Rac GTPase-activating protein; brain stress early protein Gbi; rho GTPase-activating protein 14; srGAP2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_080448.4 → NP_536696.4 SLIT-ROBO Rho GTPase-activating protein 3

See identical proteins and their annotated locations for NP_536696.4

Status: VALIDATED

Source sequence(s)

AC153591, AK051262, AY057899, CF744803, CK032058

Consensus CDS

CCDS39590.1

UniProtKB/Swiss-Prot

Q80U09, Q812A2, Q8BKP4, Q8BLD0, Q925I2

UniProtKB/TrEMBL

F8VPQ4

Related

ENSMUSP00000085712.5, ENSMUST00000088373.11

Conserved Domains (3) summary

cd07684
Location:26 → 278

F-BAR_srGAP3; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Protein 3

cd11955
Location:748 → 800

SH3_srGAP1-3; Src homology 3 domain of Slit-Robo GTPase Activating Proteins 1, 2, and 3

cd04383
Location:502 → 689

RhoGAP_srGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in srGAPs. srGAPs are components of the intracellular part of Slit-Robo signalling pathway that is important for axon guidance and cell migration. srGAPs contain an ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

112694932..112924466 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006506145.2 → XP_006506208.1 SLIT-ROBO Rho GTPase-activating protein 3 isoform X2

UniProtKB/Swiss-Prot

Q80U09, Q812A2, Q8BKP4, Q8BLD0, Q925I2

UniProtKB/TrEMBL

E9QN14

Related

ENSMUSP00000108794.3, ENSMUST00000113169.9

Conserved Domains (3) summary

cd07684
Location:26 → 278

F-BAR_srGAP3; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Protein 3

cd11955
Location:724 → 776

SH3_srGAP1-3; Src homology 3 domain of Slit-Robo GTPase Activating Proteins 1, 2, and 3

cd04383
Location:481 → 665

RhoGAP_srGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in srGAPs. srGAPs are components of the intracellular part of Slit-Robo signalling pathway that is important for axon guidance and cell migration. srGAPs contain an ...
XM_030255398.1 → XP_030111258.1 SLIT-ROBO Rho GTPase-activating protein 3 isoform X4

Conserved Domains (3) summary

cd07684
Location:26 → 278

F-BAR_srGAP3; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Protein 3

cd11955
Location:748 → 800

SH3_srGAP1-3; Src homology 3 domain of Slit-Robo GTPase Activating Proteins 1, 2, and 3

cd04383
Location:502 → 689

RhoGAP_srGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in srGAPs. srGAPs are components of the intracellular part of Slit-Robo signalling pathway that is important for axon guidance and cell migration. srGAPs contain an ...
XM_006506146.2 → XP_006506209.1 SLIT-ROBO Rho GTPase-activating protein 3 isoform X3

Conserved Domains (3) summary

cd07684
Location:26 → 278

F-BAR_srGAP3; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Protein 3

cd11955
Location:753 → 805

SH3_srGAP1-3; Src homology 3 domain of Slit-Robo GTPase Activating Proteins 1, 2, and 3

cd04383
Location:510 → 694

RhoGAP_srGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in srGAPs. srGAPs are components of the intracellular part of Slit-Robo signalling pathway that is important for axon guidance and cell migration. srGAPs contain an ...
XM_006506144.2 → XP_006506207.1 SLIT-ROBO Rho GTPase-activating protein 3 isoform X1

UniProtKB/Swiss-Prot

Q80U09, Q812A2, Q8BKP4, Q8BLD0, Q925I2

Conserved Domains (3) summary

cd07684
Location:26 → 278

F-BAR_srGAP3; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Protein 3

cd11955
Location:753 → 805

SH3_srGAP1-3; Src homology 3 domain of Slit-Robo GTPase Activating Proteins 1, 2, and 3

cd04383
Location:510 → 694

RhoGAP_srGAP; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in srGAPs. srGAPs are components of the intracellular part of Slit-Robo signalling pathway that is important for axon guidance and cell migration. srGAPs contain an ...