Cloning and expression of a novel human galectin cDNA, predominantly expressed in placenta(1)

Biochim Biophys Acta. 2002 Apr 12;1574(3):407-11. doi: 10.1016/s0167-4781(01)00319-0.

Abstract

A novel human galectin cDNA (PPL13) was isolated by screening a human 18-week fetal brain library. The mRNA was predominantly expressed in placenta, while the expression of it was not or barely detectable in heart, brain, lung, liver, skeletal muscle, kidney, and pancreas by Northern blot. COS-7 cells transfected with cDNA encoding human PPL13 sequestered the protein in nuclei although it lacked any known nuclear localization signal. STS of Unigene Hs. 24236 placed the cDNA to human chromosome 19q13.2.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / embryology
  • Brain / metabolism
  • COS Cells
  • Cell Nucleus / metabolism
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification
  • Female
  • Galectins
  • Gene Library
  • Humans
  • Molecular Sequence Data
  • Placenta / metabolism*
  • Pregnancy Proteins / biosynthesis
  • Pregnancy Proteins / chemistry
  • Pregnancy Proteins / genetics*
  • Sequence Alignment
  • Transfection

Substances

  • DNA, Complementary
  • Galectins
  • LGALS13 protein, human
  • Pregnancy Proteins

Associated data

  • GENBANK/AF267852
  • GENBANK/AF367430
  • GENBANK/AF367431
  • GENBANK/AF367432