Abstract
Using a gene trap technique, we identified a murine homologue of the yeast LUC7-like gene (Luc7l), which is a serine-arginine-rich protein (SR protein) that localizes in the nucleus through its arginine-serine-rich domain (RS domain) at the C-terminus and shows a speckled distribution pattern. Although its transcripts are widely expressed in embryos and adults, they are rarely detected in adult skeletal muscle, and Luc7l expression was found to be negatively regulated during the course of development of limb skeletal muscle, as well as during in vitro differentiation of the myoblast cell lines Sol8 and C2C12. We also demonstrated that forced expression of Luc7l protein inhibited myogenesis in vitro. Based on our results, Luc7l is thought to play an important role in the regulation of muscle differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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Blotting, Western
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COS Cells
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Cell Differentiation / genetics
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Cell Differentiation / physiology
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Cell Line
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Chlorocebus aethiops
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Down-Regulation / genetics
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Female
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Gene Expression Profiling
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Gene Expression Regulation, Developmental
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Male
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Mice
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Mice, Mutant Strains
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Microscopy, Fluorescence
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Muscle Development / genetics*
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Muscle Development / physiology
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Muscle, Skeletal / embryology
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Muscle, Skeletal / growth & development
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Muscle, Skeletal / metabolism
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Myoblasts / cytology
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Myoblasts / metabolism
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Nuclear Proteins / genetics*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA-Binding Proteins / genetics*
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RNA-Binding Proteins / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Transfection
Substances
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Luc7l protein, mouse
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Nuclear Proteins
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RNA, Messenger
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RNA-Binding Proteins
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Recombinant Fusion Proteins
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Green Fluorescent Proteins