DNMT3B7 expression related to MENT expression and its promoter methylation in human lymphomas

Lobna Alkebsi; Hiroshi Handa; Yoshiko Sasaki; Yohei Osaki; Kunio Yanagisawa; Yoshiaki Ogawa; Akihiko Yokohama; Hikaru Hattori; Hiromi Koiso; Takayuki Saitoh; Takeki Mitsui; Norifumi Tsukamoto; Yoshihisa Nojima; Hirokazu Murakami

doi:10.1016/j.leukres.2013.09.014

DNMT3B7 expression related to MENT expression and its promoter methylation in human lymphomas

Leuk Res. 2013 Dec;37(12):1662-7. doi: 10.1016/j.leukres.2013.09.014. Epub 2013 Sep 21.

Authors

Lobna Alkebsi¹, Hiroshi Handa, Yoshiko Sasaki, Yohei Osaki, Kunio Yanagisawa, Yoshiaki Ogawa, Akihiko Yokohama, Hikaru Hattori, Hiromi Koiso, Takayuki Saitoh, Takeki Mitsui, Norifumi Tsukamoto, Yoshihisa Nojima, Hirokazu Murakami

Affiliation

¹ Graduate School of Health Sciences, Gunma University, Gunma, Japan. Electronic address: lobnakibsi@gmail.com.

PMID: 24094886
DOI: 10.1016/j.leukres.2013.09.014

Abstract

DNA methyltransferase (DNMT) 3B7 is the most expressed DNMT3B splice variant. It was reported that the loss of DNMT3B function led to overexpression of the MEthylated in Normal Thymocyes (MENT) and accelerated mouse lymphomagenesis. We investigated the DNMT3B7 expression and its relationship to MENT expression and promoter methylation in human lymphomas. DNMT3B7 and MENT expression were significantly (p<0.0001, p<0.01) higher in lymphomas than in non-malignant. Expression of DNMT3B7 and MENT were associated with MENT promoter hypomethylation. DNMT3B7 overexpression might interfere with the normal DNA methylation mechanism required for silencing the MENT proto-oncogene, and may accelerate human lymphomagenesis.

Keywords: DNMT3B7; Human lymphoma; MENT.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alternative Splicing / genetics
DNA (Cytosine-5-)-Methyltransferase 1
DNA (Cytosine-5-)-Methyltransferases / genetics*
DNA Methylation / physiology*
DNA Methyltransferase 3A
DNA Methyltransferase 3B
Gene Expression Regulation, Enzymologic / physiology
Gene Expression Regulation, Neoplastic
Humans
Isoenzymes / genetics
Leukocytes, Mononuclear / metabolism
Leukocytes, Mononuclear / pathology
Lymphoma / genetics*
Lymphoma / pathology
Neoplasm Proteins / genetics*
Promoter Regions, Genetic*
Proto-Oncogene Mas
Proto-Oncogenes / genetics*

Substances

C1orf56 protein, human
DNMT3A protein, human
Isoenzymes
MAS1 protein, human
Neoplasm Proteins
Proto-Oncogene Mas
DNA (Cytosine-5-)-Methyltransferase 1
DNA (Cytosine-5-)-Methyltransferases
DNA Methyltransferase 3A