Abstract
We have used the polymerase chain reaction and VH family-based primers to clone and sequence 74 human germline VH segments from a single individual and built a directory to include all known germline sequences. The directory contains 122 VH segments with different nucleotide sequences, 83 of which have open reading frames. The directory indicates that the structural diversity of the germline repertoire for antigen binding is fixed by about 50 groups of VH segments: each group encodes identical hypervariable loops. The directory should help in mapping the VH locus, in estimating somatic mutation and VH segment usage and in designing and constructing synthetic antibody libraries.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
Cloning, Molecular
-
Gene Library
-
Gene Rearrangement, B-Lymphocyte, Heavy Chain / genetics
-
Genes, Immunoglobulin*
-
Humans
-
Immunoglobulin Heavy Chains / chemistry
-
Immunoglobulin Heavy Chains / genetics*
-
Immunoglobulin Variable Region / chemistry
-
Immunoglobulin Variable Region / genetics*
-
Molecular Sequence Data
-
Oligonucleotide Probes / genetics
-
Polymerase Chain Reaction
Substances
-
Immunoglobulin Heavy Chains
-
Immunoglobulin Variable Region
-
Oligonucleotide Probes
Associated data
-
GENBANK/Z12303
-
GENBANK/Z12304
-
GENBANK/Z12305
-
GENBANK/Z12306
-
GENBANK/Z12307
-
GENBANK/Z12308
-
GENBANK/Z12309
-
GENBANK/Z12310
-
GENBANK/Z12311
-
GENBANK/Z12312
-
GENBANK/Z12313
-
GENBANK/Z12314
-
GENBANK/Z12315
-
GENBANK/Z12316
-
GENBANK/Z12317
-
GENBANK/Z12318
-
GENBANK/Z12319
-
GENBANK/Z12320
-
GENBANK/Z12321
-
GENBANK/Z12322
-
GENBANK/Z12323
-
GENBANK/Z12324
-
GENBANK/Z12325
-
GENBANK/Z12326
-
GENBANK/Z12327
-
GENBANK/Z12328
-
GENBANK/Z12329
-
GENBANK/Z12330
-
GENBANK/Z12331
-
GENBANK/Z12332