Abstract
The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine-thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase eta.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adenine / metabolism
-
DNA Damage
-
DNA Polymerase iota
-
DNA Repair*
-
DNA, Fungal / biosynthesis
-
DNA-Directed DNA Polymerase / genetics
-
DNA-Directed DNA Polymerase / metabolism*
-
Deoxyribonucleotides / metabolism
-
Enzyme Inhibitors / pharmacology
-
Nucleic Acid Synthesis Inhibitors
-
Pyrimidine Dimers / metabolism*
-
Recombinant Fusion Proteins / metabolism
-
Saccharomyces cerevisiae / enzymology*
-
Saccharomyces cerevisiae / genetics
-
Templates, Genetic
-
Ultraviolet Rays
Substances
-
DNA, Fungal
-
Deoxyribonucleotides
-
Enzyme Inhibitors
-
Nucleic Acid Synthesis Inhibitors
-
Pyrimidine Dimers
-
Recombinant Fusion Proteins
-
DNA-Directed DNA Polymerase
-
Rad30 protein
-
Adenine
-
DNA Polymerase iota