Thermostable DNA-polymerase from Thermus thermophilus B35: isolation and characterization of some properties

Biochemistry (Mosc). 1998 Nov;63(11):1266-70.

Abstract

Thermostable Tte DNA-polymerase was isolated from the strain Thermus thermophilus B35 which was found in hot spring water. The enzyme with molecular mass 87 kD was isolated using sequential chromatography on DEAE-Sepharose, hydroxylapatite, hexyl-agarose, and heparin-Sepharose. Biochemical properties of Tte DNA-polymerase are similar to those of Tth DNA-polymerase isolated from Thermus thermophilus HB8; however, practical application of Tte-Pol seems to be more favorable due to higher temperature optimum of this enzyme and lack of restriction endonucleases in the initial strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cations, Divalent / pharmacology
  • Chromatography
  • Chromatography, Affinity
  • Chromatography, Ion Exchange
  • DNA-Directed DNA Polymerase / chemistry
  • DNA-Directed DNA Polymerase / isolation & purification*
  • DNA-Directed DNA Polymerase / metabolism*
  • Durapatite
  • Enzyme Stability
  • Hot Temperature
  • Kinetics
  • Molecular Weight
  • Thermodynamics
  • Thermus thermophilus / enzymology*

Substances

  • Cations, Divalent
  • Durapatite
  • DNA-Directed DNA Polymerase