BASH, a novel signaling molecule preferentially expressed in B cells of the bursa of Fabricius

J Immunol. 1998 Dec 1;161(11):5804-8.

Abstract

The bursa of Fabricius is a gut-associated lymphoid organ that is essential for the generation of a diversified B cell repertoire in the chicken. We describe here a novel gene preferentially expressed in bursal B cells. The gene encodes an 85-kDa protein, designated BASH (B cell adaptor containing SH2 domain), that contains N-terminal acidic domains with SH2 domain-binding phosphotyrosine-based motifs, a proline-rich domain, and a C-terminal SH2 domain. BASH shows a substantial sequence similarity to SLP-76, an adaptor protein functioning in TCR-signal transduction. BASH becomes tyrosine-phosphorylated with the B cell Ag receptor (BCR) cross-link or by coexpression with Syk and Lyn and associates with signaling molecules including Syk and a putative chicken Shc homologue. Overexpression of BASH results in suppression of the NF-AT activation induced by BCR-cross-linking. These findings suggest that BASH is involved in BCR-mediated signal transduction and could play a critical role in B cell development in the bursa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Adaptor Proteins, Vesicular Transport*
  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism*
  • Bursa of Fabricius / cytology
  • Bursa of Fabricius / immunology
  • Bursa of Fabricius / metabolism*
  • Carrier Proteins*
  • Cell Line
  • Chickens
  • DNA, Complementary / isolation & purification
  • DNA-Binding Proteins / metabolism
  • Enzyme Precursors / metabolism
  • Enzyme Precursors / physiology
  • Humans
  • Immunoglobulin M / metabolism
  • Intracellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • NFATC Transcription Factors
  • Nuclear Proteins*
  • Phosphoproteins / biosynthesis*
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Phosphorylation
  • Protein-Tyrosine Kinases / metabolism
  • Protein-Tyrosine Kinases / physiology
  • Proteins / metabolism
  • RNA, Messenger / biosynthesis
  • Receptors, Antigen, B-Cell / metabolism
  • Receptors, Antigen, B-Cell / physiology
  • Shc Signaling Adaptor Proteins
  • Signal Transduction / immunology*
  • Src Homology 2 Domain-Containing, Transforming Protein 1
  • Syk Kinase
  • Transcription Factors / metabolism
  • Tyrosine / metabolism
  • src Homology Domains / immunology*
  • src-Family Kinases / physiology

Substances

  • Adaptor Proteins, Signal Transducing
  • Adaptor Proteins, Vesicular Transport
  • B cell linker protein
  • Carrier Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • Enzyme Precursors
  • Immunoglobulin M
  • Intracellular Signaling Peptides and Proteins
  • NFATC Transcription Factors
  • Nuclear Proteins
  • Phosphoproteins
  • Proteins
  • RNA, Messenger
  • Receptors, Antigen, B-Cell
  • SHC1 protein, human
  • Shc Signaling Adaptor Proteins
  • Src Homology 2 Domain-Containing, Transforming Protein 1
  • Transcription Factors
  • Tyrosine
  • Protein-Tyrosine Kinases
  • SYK protein, human
  • Syk Kinase
  • src-Family Kinases

Associated data

  • GENBANK/AB015289