Abstract
A large number of sequence-related, cysteine-rich membrane proteins containing metalloproteinase-like and disintegrin-like domains (the MDC protein family) have been identified in mammalian tissues from a variety of species. Previous studies in the macaque (Macaca fascicularis) have led to the cDNA cloning and sequence analysis of a number of MDC proteins which are abundantly expressed in the male reproductive tract. We now describe the distribution of seven of these macaque MDC transcripts in a range of different tissues. This description includes a novel macaque testis-derived MDC, tMDC III, whose full-length sequence is reported for the first time. In addition, polyclonal antisera have been used to localize a number of these MDC proteins to spermatogenic cells in testis sections, and to demonstrate their processing on the sperm surface during epididymal transit.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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ADAM Proteins
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Amino Acid Sequence
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Animals
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Dimerization
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Endopeptidases / metabolism
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Epididymis / metabolism
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Fertilins
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Fluorescent Antibody Technique, Indirect
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Genitalia, Male / growth & development
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Genitalia, Male / metabolism*
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Guinea Pigs
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Immune Sera
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Macaca fascicularis / genetics
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Macaca fascicularis / growth & development
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Macaca fascicularis / metabolism*
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Male
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Membrane Glycoproteins / genetics*
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Membrane Glycoproteins / immunology
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Membrane Glycoproteins / metabolism
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Membrane Proteins / genetics*
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Membrane Proteins / immunology
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Membrane Proteins / metabolism
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Metalloendopeptidases / genetics*
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Metalloendopeptidases / immunology
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Metalloendopeptidases / metabolism
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Mice
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Molecular Sequence Data
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Multigene Family
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Organ Specificity
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Protein Processing, Post-Translational
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Proteins / genetics*
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Proteins / immunology
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Proteins / metabolism
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RNA, Messenger / biosynthesis
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Rats
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Sequence Alignment
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Sequence Homology, Amino Acid
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Sexual Maturation
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Species Specificity
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Spermatogenesis
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Spermatozoa / metabolism
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Testis / cytology
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Testis / metabolism
Substances
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Immune Sera
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Membrane Glycoproteins
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Membrane Proteins
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Proteins
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RNA, Messenger
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tMDC I protein, Macaca fascicularis
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tMDC IV protein, Macaca fascicularis
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Endopeptidases
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ADAM Proteins
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Adam18 protein, rat
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Adam5 protein, rat
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Adam7 protein, mouse
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Adam7 protein, rat
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Fertilins
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Metalloendopeptidases
Associated data
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GENBANK/X76637
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GENBANK/X77619
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GENBANK/X77653
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GENBANK/X79808
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GENBANK/X79809
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GENBANK/X87205
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GENBANK/Y08617