Peptide binding consensus of the NHE-RF-PDZ1 domain matches the C-terminal sequence of cystic fibrosis transmembrane conductance regulator (CFTR)

FEBS Lett. 1998 May 1;427(1):103-8. doi: 10.1016/s0014-5793(98)00402-5.

Abstract

The Na+-H+ exchanger regulatory factor (NHE-RF) is a cytoplasmic phosphoprotein that was first found to be involved in protein kinase A mediated regulation of ion transport. NHE-RF contains two distinct protein interaction PDZ domains: NHE-RF-PDZ1 and NHE-RF-PDZ2. However, their binding partners are currently unknown. Because PDZ domains usually bind to specific short linear C-terminal sequences, we have carried out affinity selection of random peptides for specific sequences that interact with the NHE-RF PDZ domains and found that NHE-RF-PDZ1 is capable of binding to the CFTR C-terminus. The specific and tight association suggests a potential regulatory role of NHE-RF in cystic fibrosis transmembrane conductance regulator (CFTR) function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Consensus Sequence
  • Cystic Fibrosis Transmembrane Conductance Regulator / chemistry
  • Cystic Fibrosis Transmembrane Conductance Regulator / genetics*
  • Cystic Fibrosis Transmembrane Conductance Regulator / metabolism
  • Drosophila Proteins*
  • Insect Proteins / chemistry
  • Mice
  • Molecular Sequence Data
  • Phosphoproteins / chemistry
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism
  • Protein Conformation
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sodium-Hydrogen Exchangers

Substances

  • Drosophila Proteins
  • Insect Proteins
  • Phosphoproteins
  • Sodium-Hydrogen Exchangers
  • cno protein, Drosophila
  • sodium-hydrogen exchanger regulatory factor
  • Cystic Fibrosis Transmembrane Conductance Regulator

Associated data

  • GENBANK/Z50150