Abstract
It was shown that verapamil and Ca(2+)-ionofor A23187 essentially inhibited the activity of RNA-polymerase in isolated nuclei of rat liver, while DNA-polymerasing activity was inhibited only after the addition of verapamil. These phenomena were not found after treatment of nuclei with 1% triton X-100. The inhibition of RNA synthesis was also found after the addition of Ca(2+)-ionofor A23187 but not verapamil to nuclei which envelope was disrupted by freezing-thawing procedure. The data obtained suggest that Ca(2+)-ionofor A23187 and verapamil affected RNA and DNA synthesis in isolated nuclei.
Publication types
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Comparative Study
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English Abstract
MeSH terms
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Animals
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Calcimycin / pharmacology*
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Calcium / metabolism
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Calcium Channel Blockers / pharmacology*
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Cell Nucleus / drug effects
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Cell Nucleus / enzymology
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DNA / biosynthesis
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DNA-Directed RNA Polymerases / antagonists & inhibitors*
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Enzyme Inhibitors / pharmacology*
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Female
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In Vitro Techniques
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Ionophores / pharmacology*
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Liver / cytology
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Liver / drug effects*
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Liver / enzymology
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Nucleic Acid Synthesis Inhibitors*
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Octoxynol
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RNA / biosynthesis
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Rats
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Verapamil / pharmacology*
Substances
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Calcium Channel Blockers
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Enzyme Inhibitors
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Ionophores
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Nucleic Acid Synthesis Inhibitors
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Calcimycin
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RNA
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Octoxynol
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DNA
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Verapamil
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DNA-Directed RNA Polymerases
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Calcium