Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase increases the efficiency of synthesis of long DNA molecules

FEBS Lett. 1998 Mar 27;425(2):249-50. doi: 10.1016/s0014-5793(98)00241-5.

Abstract

Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase (Klentaq) increases several times the efficiency of synthesis of long (over 2 kbp) DNA molecules. The difference in the DNA synthesis efficiencies by the mutant and native enzymes increased with the increase in the DNA fragment length.

MeSH terms

  • Asparagine / genetics
  • Asparagine / metabolism*
  • Bacteriophage lambda / genetics
  • DNA, Viral / biosynthesis*
  • Mutagenesis, Site-Directed*
  • Serine / genetics
  • Serine / metabolism*
  • Taq Polymerase / genetics
  • Taq Polymerase / metabolism*

Substances

  • DNA, Viral
  • Serine
  • Asparagine
  • Taq Polymerase