Abstract
We demonstrate that members of the olfactory receptor (OR) gene family are distributed on all but a few human chromosomes. Through FISH analysis, we show that OR sequences reside at more than 25 locations in the human genome. Their distribution is biased for terminal bands. Flow-sorted chromosomes were used to isolate 87 OR sequences derived from 16 chromosomes. Their sequence-relationships are indicative of the inter- and intrachromosomal duplications responsible for OR family expansion. The human genome has accumulated a striking number of dysfunctional copies: 72% of the sequences are pseudogenes. ORF-containing sequences predominate on chromosomes 7, 16 and 17.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Chromosome Mapping
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Chromosomes, Human*
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Chromosomes, Human, Pair 17
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Cloning, Molecular
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Conserved Sequence
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DNA Primers
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Genetic Techniques
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Humans
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In Situ Hybridization, Fluorescence
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Introns
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Molecular Sequence Data
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Multigene Family
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Receptors, Odorant / genetics*
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Sequence Analysis
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Sequence Homology, Amino Acid
Substances
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DNA Primers
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Receptors, Odorant
Associated data
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GENBANK/AF022649
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GENBANK/U86215
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GENBANK/U86216
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GENBANK/U86217
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GENBANK/U86218
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GENBANK/U86219
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GENBANK/U86220
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GENBANK/U86221
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GENBANK/U86222
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GENBANK/U86223
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GENBANK/U86224
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GENBANK/U86225
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GENBANK/U86226
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GENBANK/U86227
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GENBANK/U86228
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GENBANK/U86229
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GENBANK/U86230
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GENBANK/U86231
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GENBANK/U86232
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GENBANK/U86233
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GENBANK/U86234
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GENBANK/U86235
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GENBANK/U86236
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GENBANK/U86237
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GENBANK/U86238
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GENBANK/U86239
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GENBANK/U86240
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GENBANK/U86241
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GENBANK/U86242
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GENBANK/U86243