Comparable sensitivity and specificity of nested PCR and single-stage PCR using a thermally activated DNA polymerase

Biotechniques. 1998 Feb;24(2):222-4. doi: 10.2144/98242bm11.
No abstract available

MeSH terms

  • DNA Primers / chemistry
  • DNA-Directed DNA Polymerase / metabolism*
  • Enzyme Activation
  • Gene Products, env / genetics
  • Gene Products, gag / genetics
  • Gene Products, pol / genetics
  • HIV-1 / chemistry
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity*
  • Temperature

Substances

  • DNA Primers
  • Gene Products, env
  • Gene Products, gag
  • Gene Products, pol
  • DNA-Directed DNA Polymerase