EGF inhibits expression of WDNM1 and sulfated glycoprotein-2 genes in mammary epithelial cells

Biochem Biophys Res Commun. 1997 Nov 7;240(1):104-7. doi: 10.1006/bbrc.1997.7617.

Abstract

We have previously shown that expressions of ferritin heavy chain (FHC), WDNM1, and sulfated glycoprotein-2 (SGP-2) genes are induced at an involution stage of mammary gland. Here we studied the effect of lactogenic hormones and EGF on the expression of involution-induced genes in HC11 mammary epithelial cells. Insulin, dexamethasone, prolactin, and its combinations did not affect expression of the genes. When cells were cultured in growth medium containing EGF, expression of WDNM1 and SGP-2 genes was strongly inhibited in a dose- and time- dependent manner, whereas expression of FHC gene was not influenced by EGF. Results demonstrate that EGF inhibits expression of WDNM1 and SGP-2 genes in mammary epithelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Clusterin
  • Dexamethasone / pharmacology
  • Epidermal Growth Factor / pharmacology*
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism*
  • Epithelial Cells / physiology
  • Female
  • Ferritins / genetics
  • Gene Expression Regulation / drug effects*
  • Glycoproteins / antagonists & inhibitors*
  • Glycoproteins / genetics*
  • Insulin / pharmacology
  • Lactation / genetics
  • Mammary Glands, Animal / drug effects
  • Mammary Glands, Animal / metabolism*
  • Mammary Glands, Animal / physiology
  • Mice
  • Molecular Chaperones*
  • Neoplasm Proteins / antagonists & inhibitors*
  • Neoplasm Proteins / genetics*
  • Prolactin / pharmacology

Substances

  • Clu protein, mouse
  • Clusterin
  • Glycoproteins
  • Insulin
  • Molecular Chaperones
  • Neoplasm Proteins
  • Wfdc18 protein, rat
  • Epidermal Growth Factor
  • Dexamethasone
  • Prolactin
  • Ferritins