Human chromosome pellicle antibody recognizing centromere protein-C (CENP-C), the main component of the kinetochore

Y Xie; Z M Ni; J R Gu; P Wong; W Q Wu; G W Xu

doi:10.1038/cr.1997.2

Human chromosome pellicle antibody recognizing centromere protein-C (CENP-C), the main component of the kinetochore

Cell Res. 1997 Jun;7(1):13-9. doi: 10.1038/cr.1997.2.

Authors

Y Xie¹, Z M Ni, J R Gu, P Wong, W Q Wu, G W Xu

Affiliation

¹ Hong Kong University of Science and Technology, Department of Biology, Hong Kong. boyxie@usthk.ust.hk

PMID: 9261558
DOI: 10.1038/cr.1997.2

Abstract

Recently the antichromosome antisera from several scleroderma patients have been found to recognize the pellicle of metaphase and anaphase chromosomes. In order to identify the pellicle components, we used these antichromosome antisera to screen a human embryonic cDNA library. The sequences of the positive clones are identical to the cDNA gene sequence of CENP-C (centromere protein C), a human centromere autoantigen. This result suggests that CENP-C is a component of the pellicle of human metaphase and anaphase chromosomes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anaphase
Animals
Autoantibodies
Autoantigens / analysis*
Autoantigens / genetics
Chromosomal Proteins, Non-Histone / analysis*
Chromosomal Proteins, Non-Histone / genetics
Chromosomes, Human / chemistry*
Cloning, Molecular
DNA, Complementary
Fetus
Gene Library
Humans
Immune Sera
Kinetochores / chemistry
L Cells
Metaphase
Mice
Scleroderma, Systemic / immunology*

Substances

Autoantibodies
Autoantigens
Chromosomal Proteins, Non-Histone
DNA, Complementary
Immune Sera
centromere protein C