Genomic structure and chromosomal localization of GML (GPI-anchored molecule-like protein), a gene induced by p53

Genomics. 1997 May 1;41(3):477-80. doi: 10.1006/geno.1997.4680.

Abstract

Among its known functions, tumor suppressor gene p53 serves as a transcriptional regulator and mediates various signals through activation of downstream genes. We recently identified a novel gene, GML (glycosylphosphatidylinositol (GPI)-anchored molecule-like protein), whose expression is specifically induced by wildtype p53. To characterize the GML gene further, we determined 35.8 kb of DNA sequence that included a consensus binding sequence for p53 and the entire GML gene. The GML gene consists of four exons; and the p53-binding sequence is present in the 5'-flanking region. In genomic organization this gene resembles genes encoding murine Ly-6 glycoproteins, a human homologue of the Ly-6 family called RIG-E, and CD59; products of these genes, known as GPI-anchored proteins, are variously involved in signal transduction, cell-cell adhesion, and cell-matrix attachment. FISH analysis revealed that the GML gene is located on human chromosome 8q24.3. Genes encoding at least two other GPI-anchored molecules, E48 and RIG-E, are also located in this region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Adhesion / genetics
  • Cell Cycle Proteins*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 8 / genetics*
  • DNA / genetics
  • Exons
  • GPI-Linked Proteins
  • Gene Expression Regulation
  • Genes, p53*
  • Glycosylphosphatidylinositols / metabolism
  • Humans
  • In Situ Hybridization, Fluorescence
  • Introns
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins*
  • Signal Transduction / genetics

Substances

  • Cell Cycle Proteins
  • GML protein, human
  • GPI-Linked Proteins
  • Glycosylphosphatidylinositols
  • Membrane Proteins
  • Neoplasm Proteins
  • DNA

Associated data

  • GENBANK/AB000381