Abstract
The spatio-temporal expression of the basic helix-loop-helix transcription factor NSCL-2 was analyzed in the postnatal development of the murine brain by in situ hybridization. We found that NSCL-2 is transiently expressed in the cerebellum. NSCL-2 was found exclusively in the premigratory zone of the external granule layer where postmitotic neurons undergo initial stages of neuronal differentiation. NSCL-2 expression was not detected in mature neurons. This pattern of expression suggests that NSCL-2 is critical for the onset of neuronal differentiation, but not for the maintenance of the differentiated state.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Basic Helix-Loop-Helix Transcription Factors
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Cell Differentiation / physiology
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Cerebellum / cytology
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Cerebellum / growth & development
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Cerebellum / metabolism*
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DNA-Binding Proteins / genetics*
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Gene Expression Regulation, Developmental / physiology*
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Helix-Loop-Helix Motifs*
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In Situ Hybridization
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Mice
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Mice, Inbred ICR
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Nerve Tissue Proteins / genetics*
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Neurons / cytology
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Neurons / metabolism
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Time Factors
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Transcription Factors / genetics*
Substances
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Basic Helix-Loop-Helix Transcription Factors
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DNA-Binding Proteins
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Nerve Tissue Proteins
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Nhlh2 protein, mouse
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Transcription Factors