Cloning and sequencing of rat cDNA for the 41-kDa phosphoribosylpyrophosphate synthetase-associated protein has a high homology to the catalytic subunits and the 39-kDa associated protein

Biochim Biophys Acta. 1997 Jan 3;1350(1):6-10. doi: 10.1016/s0167-4781(96)00190-x.

Abstract

Rat liver phosphoribosylpyrophosphate synthetase is a complex aggregate of 34-kDa catalytic subunits (PRS I and II) and 39- and 41-kDa associated proteins (PAP39 and 41). When the rat cDNA encoding PAP41 was isolated, the deduced protein sequence was seen to contain 369 amino acids with a calculated molecular mass of 41130. PAP41 has a 79 and 49% identity with PAP39 and PRSs, respectively. When conservative substitutions are included, PAP41 and the three other components have a 66% homology. PAP41 shares some common features with PAP39 and the two proteins form the PAP subfamily. The mRNA of PAP41 is present in all rat tissues we examined.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Complementary
  • Intracellular Signaling Peptides and Proteins
  • Liver / metabolism*
  • Macromolecular Substances
  • Male
  • Molecular Sequence Data
  • Molecular Weight
  • Organ Specificity
  • Peptide Fragments / chemistry
  • Protein Biosynthesis
  • Proteins / chemistry*
  • Proteins / genetics*
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Homology, Amino Acid
  • Transcription, Genetic

Substances

  • DNA, Complementary
  • Intracellular Signaling Peptides and Proteins
  • Macromolecular Substances
  • PAP39
  • PAP41
  • Peptide Fragments
  • Proteins

Associated data

  • GENBANK/D84434