The algorithm of estimation of the Km values for primers in DNA synthesis catalyzed by human DNA polymerase alpha

T I Kolocheva; G A Maksakova; O D Zakharova; G A Nevinsky

doi:10.1016/s0014-5793(96)01298-7

The algorithm of estimation of the Km values for primers in DNA synthesis catalyzed by human DNA polymerase alpha

FEBS Lett. 1996 Dec 9;399(1-2):113-6. doi: 10.1016/s0014-5793(96)01298-7.

Authors

T I Kolocheva¹, G A Maksakova, O D Zakharova, G A Nevinsky

Affiliation

¹ Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences.

PMID: 8980132
DOI: 10.1016/s0014-5793(96)01298-7

Abstract

DNA synthesis with various deoxyribo homo- and heterooligoprimers in the presence of complementary templates was investigated. The lengthening of d(pN)n, primers (n = 1-10) by a unit resulted in an increase of the primer affinity and a maximal rate of polymerization. The coefficient of the affinity enhancement of primers due to formation of one hydrogen bond between primer and template was found to be 1.35. The dependence of the primer affinity and polymerization rate on template-primer structure in solution was analyzed and the objective laws of the changes of the KM and Vmax values were revealed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms*
Catalysis
DNA Polymerase II / metabolism*
DNA Primers*
DNA Replication*
Humans
Templates, Genetic

Substances

DNA Primers
DNA Polymerase II