Structural characterization of bovine CD36 from the milk fat globule membrane

Biochim Biophys Acta. 1996 Nov 11;1309(1-2):63-8. doi: 10.1016/s0167-4781(96)00143-1.

Abstract

Bovine CD36 from milk fat globule membranes was characterized and a full-length CD36 cDNA of 2772 nucleotides was isolated from a bovine mammary gland cDNA library. The deduced protein sequence contains 472 amino acid residues with 82-84% identity to the amino acid sequences of CD36 from other species. Peptides corresponding to 43% of the protein were sequenced. All eight potential N-glycosylation sites were glycosylated and the carbohydrate compositions of the individual sites were determined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CD36 Antigens / chemistry*
  • CD36 Antigens / genetics
  • CD36 Antigens / isolation & purification
  • Carbohydrates / analysis
  • Cattle
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • Female
  • Glycosylation
  • Mammary Glands, Animal
  • Membrane Lipids / chemistry
  • Milk / chemistry*
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Sequence Analysis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • CD36 Antigens
  • Carbohydrates
  • DNA, Complementary
  • Membrane Lipids
  • RNA, Messenger

Associated data

  • GENBANK/X91503