Abstract
Bovine CD36 from milk fat globule membranes was characterized and a full-length CD36 cDNA of 2772 nucleotides was isolated from a bovine mammary gland cDNA library. The deduced protein sequence contains 472 amino acid residues with 82-84% identity to the amino acid sequences of CD36 from other species. Peptides corresponding to 43% of the protein were sequenced. All eight potential N-glycosylation sites were glycosylated and the carbohydrate compositions of the individual sites were determined.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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CD36 Antigens / chemistry*
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CD36 Antigens / genetics
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CD36 Antigens / isolation & purification
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Carbohydrates / analysis
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Cattle
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Cloning, Molecular
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DNA, Complementary / genetics*
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Female
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Glycosylation
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Mammary Glands, Animal
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Membrane Lipids / chemistry
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Milk / chemistry*
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Molecular Sequence Data
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RNA, Messenger / analysis
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Sequence Analysis
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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CD36 Antigens
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Carbohydrates
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DNA, Complementary
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Membrane Lipids
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RNA, Messenger