Isolation of a novel GPI-anchored gene specifically regulated by p53; correlation between its expression and anti-cancer drug sensitivity

Oncogene. 1996 Nov 7;13(9):1965-70.

Abstract

We have identified a novel gene inducible by wild-type p53. A significant correlation between expression of this gene and p53 status in cells derived from esophageal cancers indicated that this gene is likely to be specifically regulated in a p53-dependent manner. As the predicted amino acid sequence showed a high degree of homology to the family of glycosyl-phosphatidylinositol (GPI)-anchored membrane proteins, we termed this gene GML (GPI-anchored molecule-like protein). Introduction of GML cDNA suppressed the growth of esophageal cancer cells in culture. A correlation between the presence of GML expression and the sensitivity of esophageal cancer cells to anti-cancer drugs implied that the gene product plays a significant role in the apoptotic pathway or cell-cycle regulation induced by p53 after DNA damage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibiotics, Antineoplastic / pharmacology
  • Bleomycin / pharmacology
  • Cell Cycle Proteins*
  • Cloning, Molecular
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / metabolism
  • Colony-Forming Units Assay
  • Drug Resistance, Neoplasm / genetics*
  • Esophageal Neoplasms / drug therapy
  • Esophageal Neoplasms / genetics
  • GPI-Linked Proteins
  • Gene Expression Regulation, Neoplastic
  • Genes, p53*
  • Glycosylphosphatidylinositols / metabolism*
  • Humans
  • Membrane Proteins / genetics*
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Neoplasm Proteins*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured

Substances

  • Antibiotics, Antineoplastic
  • Cell Cycle Proteins
  • GML protein, human
  • GPI-Linked Proteins
  • Glycosylphosphatidylinositols
  • Membrane Proteins
  • Neoplasm Proteins
  • Bleomycin

Associated data

  • GENBANK/AB000381
  • GENBANK/D64137
  • GENBANK/D84290