Small Maf proteins interact with the human transcription factor TCF11/Nrf1/LCR-F1

Nucleic Acids Res. 1996 Nov 1;24(21):4289-97. doi: 10.1093/nar/24.21.4289.

Abstract

The human TCF11 gene encodes a ubiquitously expressed bZIP transcription factor of the cap n' collar (CNC) domain family. It has a high sequence similarity to the erythroid-specific bZIP factor p45 NF-E2 in the CNC domain, which is involved in DNA binding. LCR-F1, a TCF11 isoform, is a more potent transcriptional activator than p45 NF-E2 in erythroid cells. We show here that the TCF11 protein interacts to form heterodimers with small Maf proteins, previously shown to dimerize with p45 NF-E2, ECH and Fos. Such heterodimerization significantly alters the DNA binding characteristics of TCF11. While TCF11 alone binds in vitro to the tandem NF-E2 site derived from 5' DNase hypersensitive site 2 in the beta-globin locus control region and to the single NF-E2 site in the porphobilinogen deaminase gene promoter, stronger binding is detected in the presence of small Maf proteins. Using antibodies, TCF11 isoforms bound to the single NF-E2 site were detected in K562 erythroid cell nuclear extracts. These findings place TCF11 as a good candidate for the proposed widely expressed factor(s) known to interact with small Maf proteins and bind NF-E2 sites in a sequence-specific manner resembling NF-E2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Avian Proteins*
  • Binding Sites
  • DNA / metabolism
  • DNA-Binding Proteins / metabolism*
  • Erythroid-Specific DNA-Binding Factors
  • Humans
  • Hydroxymethylbilane Synthase / genetics
  • Immunoassay
  • Leucine Zippers
  • MafB Transcription Factor
  • MafF Transcription Factor
  • NF-E2 Transcription Factor
  • NF-E2 Transcription Factor, p45 Subunit
  • NF-E2-Related Factor 1
  • Nuclear Proteins / metabolism
  • Nuclear Respiratory Factor 1
  • Nuclear Respiratory Factors
  • Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-maf
  • Trans-Activators / metabolism
  • Transcription Factors / metabolism*

Substances

  • Avian Proteins
  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • MAF protein, human
  • MAFB protein, human
  • MAFF protein, Gallus gallus
  • MAFF protein, human
  • MafB Transcription Factor
  • MafF Transcription Factor
  • NF-E2 Transcription Factor
  • NF-E2 Transcription Factor, p45 Subunit
  • NF-E2-Related Factor 1
  • NFE2 protein, human
  • NFE2L1 protein, human
  • NRF1 protein, human
  • Nuclear Proteins
  • Nuclear Respiratory Factor 1
  • Nuclear Respiratory Factors
  • Oncogene Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-maf
  • Trans-Activators
  • Transcription Factors
  • DNA
  • Hydroxymethylbilane Synthase