The nucleotide sequence of the bovine interleukin-5-encoding cDNA

Gene. 1996 Oct 17;176(1-2):273-4. doi: 10.1016/0378-1119(96)00252-1.

Abstract

The nucleotide (nt) sequence encoding the bovine homologue of interleukin-5 (boIL-5) was determined. Total cDNA generated from stimulated bovine peripheral blood lymphocytes was used to amplify the boIL-5 gene using primers based on the 5' and 3' untranslated regions of the ovine IL-5 cDNA sequence. The boIL-5 coding sequence is 405 bp long, coding for a 15.2 kDa precursor protein of 134 amino acids (aa). Cleavage of a putative signal peptide of 19 aa yields a mature form of 13.1 kDa. Comparisons at the nt level revealed 96%, 81%, 74% and 73% identity with the ovine, human, mouse and rat IL-5 sequences, respectively, and 97%, 66%, 59% and 58% aa identity, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • DNA, Complementary
  • Humans
  • Interleukin-5 / genetics*
  • Molecular Sequence Data
  • Sequence Analysis, DNA*

Substances

  • DNA, Complementary
  • Interleukin-5

Associated data

  • GENBANK/Z67872