Identification and characterization of novel genes modulated in the thyroid of dogs treated with methimazole and propylthiouracil

J Biol Chem. 1996 Nov 8;271(45):28451-7. doi: 10.1074/jbc.271.45.28451.

Abstract

Induction of cell proliferation by mitogen or growth factor stimulation leads to the specific stimulation or repression of a large number of genes. To better understand differentiated epithelial cell growth regulation, we have initiated a study to identify genes which are regulated by the thyrotropin-dependent mitogenic pathway in dog thyroid cells. A thyroid cDNA library was prepared from a methimazole and propylthiouracil-treated dog and differentially screened with probes derived from control or stimulated thyroids. Among 19 clones isolated, 6 encode known proteins (inwardly rectifying potassium channel, nucleosome assembly protein, ribosomal protein L7, elongation factor 1alpha, non-muscle myosin light chain, and heat shock protein 90beta). The 13 others correspond to proteins whose function is unknown. Among them, 5 correspond to mRNAs whose expression was modulated by mitogenic stimulation of thyrocytes in primary culture. A preliminary characterization of two of these cDNAs is reported: clone 5, which might represent a novel, atypical protein kinase, and clone 3, which contains ankyrin-like repeats, suggesting that it might interact with other proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antithyroid Agents / pharmacology*
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • Dogs
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation / drug effects*
  • HSP90 Heat-Shock Proteins / genetics
  • Methimazole / pharmacology*
  • Molecular Sequence Data
  • Propylthiouracil / pharmacology*
  • RNA, Messenger / metabolism
  • Thyroid Gland / drug effects*
  • Thyrotropin / genetics

Substances

  • Antithyroid Agents
  • HSP90 Heat-Shock Proteins
  • RNA, Messenger
  • Methimazole
  • Propylthiouracil
  • Thyrotropin

Associated data

  • GENBANK/X99144
  • GENBANK/X99145