A Candida albicans gene encoding a DNA ligase. [Yeast. 1996]

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1: Yeast. 1996 Jul;12(9):893-8.Links

A Candida albicans gene encoding a DNA ligase.

Andaluz E, Larriba G, Calderone R.

Departamento de Microbiologia, F. Ciencias, Universidad de Extremadura, Badajoz, Spain.

A DNA ligase-encoding gene (Ca CDC9) was cloned from Candida albicans by complementation of an ime-1 mutation in Saccharomyces cerevisiae. In this system, IME1 function was assayed using a S. cerevisiae strain with a ime2-promoter-lacZ gene fusion such that following transformation with a C. albicans genomic library, the presence of positive clones was indicated upon the addition of X-gal to sporulation media. Transforming fragments were subcloned in pGEM7 and sequenced. Sequence homology with several ATP-dependent DNA ligases from viruses, fission yeast, human, baker yeast and bacteria was observed.

PMID: 8840507 [PubMed - indexed for MEDLINE]

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A Candida albicans gene encoding a DNA ligase.

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