Abstract
The in vitro relationship between eukaryotic DNA polymerases and fatty acids was investigated. Some fatty acids strongly inhibited the activities of DNA polymerase alpha and/or beta in vitro. The kinetics of inhibition by linoleic acid showed that DNA polymerase alpha was non-competitively inhibited with respect to the DNA template and substrate (dTTP), while DNA polymerase beta was inhibited competitively with both DNA and substrate.
MeSH terms
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Animals
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Cattle
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DNA Polymerase I / antagonists & inhibitors
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DNA Polymerase I / metabolism
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DNA Polymerase II / antagonists & inhibitors
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DNA Polymerase II / metabolism
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DNA-Directed DNA Polymerase / metabolism
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Detergents / pharmacology
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Fatty Acids / chemistry
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Fatty Acids / pharmacology*
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Kinetics
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Linoleic Acid
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Linoleic Acids / pharmacology
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Nucleic Acid Synthesis Inhibitors*
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Octoxynol
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Polyethylene Glycols / pharmacology
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Rats
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Thymine Nucleotides / metabolism
Substances
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Detergents
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Fatty Acids
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Linoleic Acids
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Nucleic Acid Synthesis Inhibitors
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Thymine Nucleotides
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Polyethylene Glycols
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Octoxynol
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Nonidet P-40
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Linoleic Acid
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DNA Polymerase I
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DNA Polymerase II
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DNA-Directed DNA Polymerase
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thymidine 5'-triphosphate