Fatty acids selectively inhibit eukaryotic DNA polymerase activities in vitro

Biochim Biophys Acta. 1996 Sep 11;1308(3):256-62. doi: 10.1016/0167-4781(96)00121-2.

Abstract

The in vitro relationship between eukaryotic DNA polymerases and fatty acids was investigated. Some fatty acids strongly inhibited the activities of DNA polymerase alpha and/or beta in vitro. The kinetics of inhibition by linoleic acid showed that DNA polymerase alpha was non-competitively inhibited with respect to the DNA template and substrate (dTTP), while DNA polymerase beta was inhibited competitively with both DNA and substrate.

MeSH terms

  • Animals
  • Cattle
  • DNA Polymerase I / antagonists & inhibitors
  • DNA Polymerase I / metabolism
  • DNA Polymerase II / antagonists & inhibitors
  • DNA Polymerase II / metabolism
  • DNA-Directed DNA Polymerase / metabolism
  • Detergents / pharmacology
  • Fatty Acids / chemistry
  • Fatty Acids / pharmacology*
  • Kinetics
  • Linoleic Acid
  • Linoleic Acids / pharmacology
  • Nucleic Acid Synthesis Inhibitors*
  • Octoxynol
  • Polyethylene Glycols / pharmacology
  • Rats
  • Thymine Nucleotides / metabolism

Substances

  • Detergents
  • Fatty Acids
  • Linoleic Acids
  • Nucleic Acid Synthesis Inhibitors
  • Thymine Nucleotides
  • Polyethylene Glycols
  • Octoxynol
  • Nonidet P-40
  • Linoleic Acid
  • DNA Polymerase I
  • DNA Polymerase II
  • DNA-Directed DNA Polymerase
  • thymidine 5'-triphosphate