Widespread expression of human cysteine string proteins

FEBS Lett. 1996 Aug 12;391(3):269-72. doi: 10.1016/0014-5793(96)00750-8.

Abstract

We present the nucleotide and deduced amino acid sequence of a human systeine string protein (csp) and a unique truncated csp variant that derives from the retention of an exonic sequence that introduces a premature, in-frame stop codon. Low stringency Southern analysis is compatible with the presence of a single human csp gene. Northern analysis reveals that human csp mRNA has both a more heterogeneous size distribution and a more widespread tissue distribution than previously reported for other csps. Exemplifying this is the fact that csp immunoreactivity is detected in Triton X-114 extracts of human blood, an observation which may facilitate blood-based diagnostic assays of csp status in man.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cysteine / chemistry*
  • DNA, Complementary / analysis
  • HSP40 Heat-Shock Proteins
  • Humans
  • Membrane Proteins*
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Open Reading Frames / genetics
  • RNA, Messenger / analysis
  • Restriction Mapping

Substances

  • DNA, Complementary
  • HSP40 Heat-Shock Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • cysteine string protein
  • Cysteine