Abstract
We describe here the cloning of canine IL-10 cDNA (GenBank accession No. U33843) and the expression of recombinant IL-10 in a dog kidney cell line (DK6247) and Chinese hamster ovary cells (CHO). Canine IL-10 exhibits strong sequence homology to the known sequences of human, mouse, rat, and bovine genes at nucleotide and amino acid levels. The IL-10 gene, when introduced into DK and CHO cell lines, produces recombinant IL-10 that causes an inhibitory effect on allogeneic MHC-driven lymphoproliferative responses.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
CHO Cells
-
Cells, Cultured
-
Cloning, Molecular
-
Cricetinae
-
DNA, Complementary / isolation & purification
-
Dogs
-
Gene Expression
-
Humans
-
Immunohistochemistry
-
Interleukin-10 / biosynthesis
-
Interleukin-10 / genetics*
-
Kidney / cytology
-
Kidney / metabolism
-
Molecular Sequence Data
-
Recombinant Proteins / biosynthesis
-
Sequence Homology, Amino Acid
-
Transfection
Substances
-
DNA, Complementary
-
Recombinant Proteins
-
Interleukin-10