Abstract
We investigated nuclease activities associated with the catalytic subunit of herpes simplex virus type 1 DNA polymerase. We confirm that a 3'-5' exonuclease copurifies with this enzyme. Previous reports suggested that a 5' DNase was intrinsic to the polymerase. Our preparation lacks such activity.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Line
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DNA-Directed DNA Polymerase / genetics
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DNA-Directed DNA Polymerase / isolation & purification
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DNA-Directed DNA Polymerase / metabolism*
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Exodeoxyribonuclease V
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Exodeoxyribonucleases / genetics
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Exodeoxyribonucleases / isolation & purification
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Exodeoxyribonucleases / metabolism*
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Herpesvirus 1, Human / enzymology*
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Humans
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Mutation
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Spodoptera / cytology
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Substrate Specificity
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Viral Proteins*
Substances
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Viral Proteins
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DNA-Directed DNA Polymerase
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Exodeoxyribonucleases
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DNA polymerase, Simplexvirus
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Exodeoxyribonuclease V