Abstract
We report here the identification of a cDNA encoding a human thiazide-sensitive sodium-chloride cotransporter (hTSC) using a PCR-based method. The homology of the hTSC with rat TSC (rTSC) and rat bumetanide-sensitive sodium-potassium-chloride cotransporter (rBSC) was 86% and 64%, respectively, at the nucleotide level, and 92% and 61%, respectively, at the amino acid level. Using fluorescence in situ hybridization (FISH), the hTSC gene has been mapped to chromosome 16q13. Northern blot analysis using polyA+RNA from various human tissues, revealed a major 4.5 kb transcript and a minor 6.5 kb specifically in the kidney, but low level of expression was also observed in small intestine, placenta, prostate, colon, and spleen.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Benzothiadiazines
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Carrier Proteins / biosynthesis*
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Carrier Proteins / chemistry*
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Carrier Proteins / genetics
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Chromosome Mapping
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Chromosomes, Human, Pair 16
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Cloning, Molecular
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Colon / metabolism
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DNA Primers
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DNA, Complementary
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Diuretics
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Female
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Flounder
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Humans
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In Situ Hybridization, Fluorescence
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Intestine, Small / metabolism
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Kidney / metabolism
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Male
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Molecular Sequence Data
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Organ Specificity
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Placenta / metabolism
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Polymerase Chain Reaction
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Pregnancy
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Prostate / metabolism
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RNA, Messenger / biosynthesis
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Rats
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Receptors, Drug / biosynthesis*
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Receptors, Drug / chemistry*
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Receptors, Drug / genetics
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Sodium Chloride Symporter Inhibitors / metabolism
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Sodium Chloride Symporters
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Solute Carrier Family 12, Member 3
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Spleen / metabolism
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Symporters*
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Transcription, Genetic*
Substances
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Benzothiadiazines
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Carrier Proteins
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DNA Primers
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DNA, Complementary
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Diuretics
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RNA, Messenger
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Receptors, Drug
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SLC12A3 protein, human
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Slc12a3 protein, rat
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Sodium Chloride Symporter Inhibitors
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Sodium Chloride Symporters
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Solute Carrier Family 12, Member 3
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Symporters
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thiazide receptor