Identification of a cDNA encoding a thiazide-sensitive sodium-chloride cotransporter from the human and its mRNA expression in various tissues

Biochem Biophys Res Commun. 1996 Jun 14;223(2):324-8. doi: 10.1006/bbrc.1996.0893.

Abstract

We report here the identification of a cDNA encoding a human thiazide-sensitive sodium-chloride cotransporter (hTSC) using a PCR-based method. The homology of the hTSC with rat TSC (rTSC) and rat bumetanide-sensitive sodium-potassium-chloride cotransporter (rBSC) was 86% and 64%, respectively, at the nucleotide level, and 92% and 61%, respectively, at the amino acid level. Using fluorescence in situ hybridization (FISH), the hTSC gene has been mapped to chromosome 16q13. Northern blot analysis using polyA+RNA from various human tissues, revealed a major 4.5 kb transcript and a minor 6.5 kb specifically in the kidney, but low level of expression was also observed in small intestine, placenta, prostate, colon, and spleen.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Benzothiadiazines
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / chemistry*
  • Carrier Proteins / genetics
  • Chromosome Mapping
  • Chromosomes, Human, Pair 16
  • Cloning, Molecular
  • Colon / metabolism
  • DNA Primers
  • DNA, Complementary
  • Diuretics
  • Female
  • Flounder
  • Humans
  • In Situ Hybridization, Fluorescence
  • Intestine, Small / metabolism
  • Kidney / metabolism
  • Male
  • Molecular Sequence Data
  • Organ Specificity
  • Placenta / metabolism
  • Polymerase Chain Reaction
  • Pregnancy
  • Prostate / metabolism
  • RNA, Messenger / biosynthesis
  • Rats
  • Receptors, Drug / biosynthesis*
  • Receptors, Drug / chemistry*
  • Receptors, Drug / genetics
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Sodium Chloride Symporter Inhibitors / metabolism
  • Sodium Chloride Symporters
  • Solute Carrier Family 12, Member 3
  • Spleen / metabolism
  • Symporters*
  • Transcription, Genetic*

Substances

  • Benzothiadiazines
  • Carrier Proteins
  • DNA Primers
  • DNA, Complementary
  • Diuretics
  • RNA, Messenger
  • Receptors, Drug
  • SLC12A3 protein, human
  • Slc12a3 protein, rat
  • Sodium Chloride Symporter Inhibitors
  • Sodium Chloride Symporters
  • Solute Carrier Family 12, Member 3
  • Symporters
  • thiazide receptor