Abstract
BMP-3b (also termed GDF-10) is a novel BMP-3 related protein recently discovered in rat femur tissue by molecular cloning. In this paper, we have isolated cDNA and the gene for human BMP-3b and determined their structure. Cloned human BMP-3b cDNA with a size of 2632 base pairs encodes a 478 amino acid precursor protein sharing 83% identity with rat BMP-3b. The human BMP-3b gene is composed of 3 exons and spans approximately 13 kilobases of DNA. The 5' flanking region carries no typical TATA box but G+C rich sequences. Southern blot analysis revealed that the BMP-3b gene is situated in a single locus of chromosome 10. By Northern analysis, human BMP-3b transcripts were detected primarily in femur, brain, lung, skeletal muscle, pancreas and testis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Bone Morphogenetic Protein 3
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Bone Morphogenetic Proteins*
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Bone and Bones / metabolism
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Brain / metabolism
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Chromosome Mapping
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Chromosomes, Human, Pair 10*
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Cloning, Molecular
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DNA Primers
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DNA, Complementary
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Exons
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Growth Differentiation Factor 10
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Growth Substances / biosynthesis*
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Growth Substances / genetics*
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Humans
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Lung / metabolism
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Male
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Molecular Sequence Data
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Muscle, Skeletal / metabolism
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Pancreas / metabolism
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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Rats
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Regulatory Sequences, Nucleic Acid
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Restriction Mapping
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TATA Box
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Testis / metabolism
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Transcription, Genetic
Substances
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BMP3 protein, human
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Bmp3 protein, rat
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Bone Morphogenetic Protein 3
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Bone Morphogenetic Proteins
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DNA Primers
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DNA, Complementary
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GDF10 protein, human
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Gdf10 protein, rat
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Growth Differentiation Factor 10
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Growth Substances
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RNA, Messenger
Associated data
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GENBANK/D49492
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GENBANK/D49493