Thymine-thymine dimer bypass by yeast DNA polymerase zeta

Science. 1996 Jun 14;272(5268):1646-9. doi: 10.1126/science.272.5268.1646.

Abstract

The REV3 and REV7 genes of the yeast Saccharomyces cerevisiae are required for DNA damage-induced mutagenesis. The Rev3 and Rev7 proteins were shown to form a complex with DNA polymerase activity. This polymerase replicated past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally severely inhibits replication, with an efficiency of approximately 10 percent. In contrast, bypass replication efficiency with yeast DNA polymerase alpha was no more than 1 percent. The Rev3-Rev7 complex is the sixth eukaryotic DNA polymerase to be described, and is therefore called DNA polymerase zeta.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Primers
  • DNA Replication*
  • DNA, Fungal / biosynthesis
  • DNA, Fungal / metabolism
  • DNA-Directed DNA Polymerase / genetics
  • DNA-Directed DNA Polymerase / metabolism*
  • Fungal Proteins / antagonists & inhibitors
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Molecular Sequence Data
  • Nucleic Acid Synthesis Inhibitors
  • Pyrimidine Dimers / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*

Substances

  • DNA Primers
  • DNA, Fungal
  • Fungal Proteins
  • Nucleic Acid Synthesis Inhibitors
  • Pyrimidine Dimers
  • REV7 protein, S cerevisiae
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Glutathione Transferase
  • DNA-Directed DNA Polymerase
  • REV3 protein, S cerevisiae