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1: Mol Cell Biol. 1996 Jun;16(6):2848-56.Click here to read Click here to read Links

Identification and analysis of a functional human homolog of the SPT4 gene of Saccharomyces cerevisiae.

Hartzog GA, Basrai MA, Ricupero-Hovasse SL, Hieter P, Winston F.

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

Spt4p is a nonhistone protein of Saccharomyces cerevisiae that is believed to be required for normal chromatin structure and transcription. In this work we describe the isolation and analysis of a human gene, SUPT4H, that encodes a predicted protein 42% identical to Spt4p. When expressed in S. cerevisiae, SUPT4H complemented all spt4 mutant phenotypes. In human cells SUPT4H encodes a nuclear protein that is expressed in all tissues tested. In addition, hybridization analyses suggest that an SUPT4H-related gene is also present in mice. SUPT4H was localized to human chromosome 17 by PCR analysis of a human-rodent somatic cell hybrid panel. Thus, like other proteins that are components of or control the structure of chromatin, Spt4p appears to be conserved from S. cerevisiae to mammals.

PMID: 8649394 [PubMed - indexed for MEDLINE]

PMCID: PMC231277

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