MafB is an interaction partner and repressor of Ets-1 that inhibits erythroid differentiation

Cell. 1996 Apr 5;85(1):49-60. doi: 10.1016/s0092-8674(00)81081-8.

Abstract

Using a yeast one-hybrid screen with a DNA-bound Ets-1 protein, we have identified MafB, an AP-1 like protein, as a direct interaction partner. MafB is specifically expressed in myelomonocytic cells and binds to the DNA-binding domain of Ets-1 via its basic region or leucine-zipper domain. Furthermore, it represses Ets-1 transactivation of synthetic promoters containing Ets binding sites and inhibits Ets-1-mediated transactivation of the transferrin receptor, which is known to be essential for erythroid differentiation. Accordingly, overexpression of MafB in an erythroblast cell line down-regulates the endogenous transferrin receptor gene and inhibits differentiation without affecting cell proliferation. These results highlight the importance of inhibitory interactions between transcription factors in regulating lineage-specific gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Avian Proteins*
  • Base Sequence
  • Binding Sites / physiology
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology
  • Chick Embryo
  • Clone Cells / physiology
  • DNA, Complementary / physiology
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / physiology
  • Erythroblasts / cytology*
  • Erythroblasts / physiology
  • G-Box Binding Factors
  • Gene Expression Regulation / physiology
  • Genetic Testing
  • Hematopoiesis / genetics
  • Macrophages / physiology
  • MafB Transcription Factor
  • Molecular Sequence Data
  • Oncogene Proteins / genetics*
  • Oncogene Proteins / metabolism
  • Protein-Tyrosine Kinases / metabolism*
  • Proto-Oncogene Protein c-ets-1
  • Proto-Oncogene Proteins / antagonists & inhibitors
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-ets
  • Receptors, Transferrin / genetics
  • Repressor Proteins / genetics
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transcription Factors / antagonists & inhibitors
  • Transcription Factors / metabolism*
  • Vertebrates
  • Yeasts / genetics

Substances

  • Avian Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • G-Box Binding Factors
  • MafB Transcription Factor
  • Oncogene Proteins
  • Proto-Oncogene Protein c-ets-1
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-ets
  • Receptors, Transferrin
  • Repressor Proteins
  • Trans-Activators
  • Transcription Factors
  • maf-B protein, chicken
  • Protein-Tyrosine Kinases

Associated data

  • GENBANK/X96511