The flavohaemoglobin (HMP) of Escherichia coli gen...[FEBS Lett. 1996]

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1: FEBS Lett. 1996 Mar 11;382(1-2):141-4. Links

The flavohaemoglobin (HMP) of Escherichia coli generates superoxide in vitro and causes oxidative stress in vivo.

Membrillo-Hernández J, Ioannidis N, Poole RK.

Division of Life Sciences, King's College London, U.K.

Purified flavohaemoglobin (HMP) of Escherichia coli reduces Fe(III) in a superoxide dismutase (SOD)-sensitive reaction, demonstrating superoxide anion generation during aerobic NADH oxidation. In vivo, sodA-lacZ fusion activity was increased 3-fold by introducing plasmid pPL341, containing the hmp gene, or by growth with paraquat. The effects were additive and SOXS-dependent. Thus HMP activity causes oxidative stress in vivo. Activities of sodA-lacZ and hmp-lacZ fusions were stimulated in a himA mutant, demonstrating repression of both promoters by integration host factor (IHF), but the effects of pPL341 on sodA-lacZ activity were not due to titration of IHF by the hmp promoter.

PMID: 8612736 [PubMed - indexed for MEDLINE]

Response of the NAD(P)H-oxidising flavohaemoglobin (Hmp) to prolonged oxidative stress and implications for its physiological role in Escherichia coli. [FEMS Microbiol Lett. 1998]
The flavohemoglobin of Escherichia coli confers resistance to a nitrosating agent, a "Nitric oxide Releaser," and paraquat and is essential for transcriptional responses to oxidative stress. [J Biol Chem. 1999]
Escherichia coli Hmp, an "oxygen-binding flavohaemoprotein", produces superoxide anion and self-destructs. [Arch Microbiol. 2004]
ReviewRegulation of sod genes in Escherichia coli: relevance to superoxide dismutase function. [Mol Microbiol. 1991]
ReviewSensing and protecting against superoxide stress in Escherichia coli--how many ways are there to trigger soxRS response? [Redox Rep. 2000]

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The flavohaemoglobin (HMP) of Escherichia coli generates superoxide in vitro and causes oxidative stress in vivo.

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