Purification of Escherichia coli DNA polymerase I and Klenow fragment

Methods Enzymol. 1995:262:3-13. doi: 10.1016/0076-6879(95)62003-6.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromatography, Gel / methods
  • Chromatography, Ion Exchange / methods
  • Cloning, Molecular
  • DNA Polymerase I / biosynthesis
  • DNA Polymerase I / isolation & purification*
  • DNA Polymerase I / metabolism
  • Enzyme Induction
  • Escherichia coli / enzymology*
  • Indicators and Reagents
  • Nalidixic Acid / pharmacology
  • Peptide Fragments / biosynthesis
  • Peptide Fragments / isolation & purification*
  • Peptide Fragments / metabolism
  • Plasmids
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism

Substances

  • Indicators and Reagents
  • Peptide Fragments
  • Recombinant Proteins
  • Nalidixic Acid
  • DNA Polymerase I