Abstract
N-(2-Deoxy-beta-D-erythro-pentofuranosyl)-N-3-(2R-hydroxyisobutyric acid)urea (alpha-R-hydroxy-beta-ureidoisobutyric acid, 8) was site specifically incorporated into a series of oligonucleotides via the ammonolysis of biopolymers containing 5R-thymidine C5-hydrate (3). alpha-R-hydroxy-beta-ureidoisobutyric acid (8) inhibits snake venom phosphodiesterase, lambda exonuclease and Klenow (exo-) fragment. Kinetic measurements for insertion of nucleotides opposite 8 by Klenow (exo-) fragment indicate that this lesion is instructive.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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DNA Polymerase I / metabolism*
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Exodeoxyribonucleases / metabolism*
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Kinetics
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Molecular Sequence Data
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Oligodeoxyribonucleotides / chemistry*
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Oligodeoxyribonucleotides / metabolism*
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Phosphodiesterase I
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Phosphoric Diester Hydrolases / metabolism*
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Structure-Activity Relationship
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Substrate Specificity
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Thymidine / analogs & derivatives*
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Thymidine / chemistry
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Viral Proteins
Substances
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Oligodeoxyribonucleotides
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Viral Proteins
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thymidine C5-hydrate
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DNA Polymerase I
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Exodeoxyribonucleases
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exo protein, Bacteriophage lambda
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Phosphoric Diester Hydrolases
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Phosphodiesterase I
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phosphodiesterase I, snake venom
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Thymidine